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Merck
CN

A7906

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, pH 7, ≥98%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.27

生物来源

bovine

质量水平

检测方案

≥98%

形式

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

poly bottle of

来源

USA origin

技术

blood typing: suitable
microbiological culture: suitable

杂质

≤5.0% Loss on drying

缺失

≤5%

pH值(酸碱度)

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

BT virus, none detected
VSV virus, none detected

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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应用

牛血清白蛋白已用于:
  • 作为 PCR(聚合酶链反应)混合物的成分
  • 分离的皮层神经元的制备
  • 免疫荧光标记
  • 作为用于牛组织消化的无 Ca2+ 盐水(含有胶原酶、透明质酸酶和 DNA 酶)的成分

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级制备

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

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分析证书(COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

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  1. Is Product A7906, Albumin from bovine serum (BSA), suitable for use as a blocking agent in ELISA?

    We have not tested the use of A7906 in ELISA. Because the remainder of the product is mostly globulins, we would not suggest A7906 for ELISA use.

  2. Is Product A7906, Albumin from bovine serum (BSA), protease-free?

    We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.

  3. Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?

    We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).

  4. Is Product A7906, Albumin from bovine serum (BSA), tested for insulin levels?

    A7906 is not tested in quality control for insulin levels. One BSA that is indicated as "suitable for insulin RIA procedures" is A7888.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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