所有图片(2)
About This Item
线性分子式:
C6H4(NH2)2·2HCl
CAS号:
分子量:
181.06
Beilstein:
3912045
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
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一般描述
邻苯二胺(o-PD)是一种无色的非荧光分子。
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
应用
在酶联免疫吸附试验(ELISA)中，邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。
重悬
将一片药片溶于 pH 5.0 的 0.05M 磷酸盐-柠檬酸盐缓冲液中至所需浓度（通常使用0.4mg/ml 的 OPD 浓度）。使用前，在每 100ml 底物缓冲溶液中加入 40 μl 新鲜的 30％ 过氧化氢，现配现用。片剂分别包装在箔包内。
警示用语：
Warning
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Rafaella P M Guimarães-Peixoto et al.
Veterinary parasitology, regional studies and reports, 20, 100375-100375 (2020-05-26)
Bovine cysticercosis remains as one of the most important cause of carcasses and viscera condemnation in Brazilian slaughterhouses. However, the efficiency of post-mortem inspection for the diagnosis of this zoonotic disease is relatively low, and few available studies were performed
Henry Surendra et al.
Malaria journal, 18(1), 227-227 (2019-07-10)
Analysis of anti-malarial antibody responses has the potential to improve characterization of the variation in exposure to infection in low transmission settings, where conventional measures, such as entomological estimates and parasitaemia point prevalence become less sensitive and expensive to measure.
Sara Buezo Montero et al.
Frontiers in cellular and infection microbiology, 10, 377-377 (2020-08-28)
Mosquitoes of the Aedes genus transmit arboviruses of great importance to human health as dengue, chikungunya, Zika and yellow fever. The tiger mosquito Aedes albopictus can play an important role as arboviral vector, especially when Aedes aegypti is absent or
Rama Devudu Puligedda et al.
Antibodies (Basel, Switzerland), 9(1) (2020-03-04)
Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains. We sought to produce IgA monoclonal antibodies (mAbs) with PV neutralizing activity. We first
Saima Ajaz et al.
American journal of physiology. Cell physiology, 320(1), C57-C65 (2020-11-06)
The COVID-19 pandemic has been the primary global health issue since its outbreak in December 2019. Patients with metabolic syndrome suffer from severe complications and a higher mortality rate due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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