一般描述
磷酸柠檬酸缓冲液是一种常用的缓冲液，与可溶性辣根过氧化物酶底物一起使用，如邻苯二胺二盐酸（OPD）或四甲基联苯胺（TMB）。它的pH值为5.0，具有出色的缓冲能力。柠檬酸磷酸盐缓冲片可配制0.05 M、pH 5.0的柠檬酸磷酸盐缓冲液。这些片剂提供了一种快速、方便、准确的合成柠檬酸磷酸酯缓冲溶液的方法。它消除了在缓冲液制备中称重单个成分的耗时和繁琐的过程。磷酸盐-柠檬酸缓冲片由最高质量的成分制造，以严格的物理和化学规格，以确保性能和批次间的一致性。pH 5.0的磷酸盐-柠檬酸盐缓冲液片剂已在免疫测定中配制为底物缓冲液。
应用
- Characterization of nettle leaves (Urtica dioica) as a novel source of protease for clotting dromedary milk by non-destructive methods.: This study uses phosphate-citrate buffer in the enzymatic extraction processes to maintain stability and activity of plant-derived enzymes, demonstrating its crucial role in the development of natural coagulants for dairy products (Bouazizi et al., 2022).
- A rapid, low pH, nutrient stress, assay to determine the bactericidal activity of compounds against non-replicating Mycobacterium tuberculosis.: Utilizing phosphate-citrate buffer, this study develops a low pH nutrient stress assay for evaluating the efficacy of antibacterial agents against dormant tuberculosis bacteria, providing a critical tool for drug development (Early et al., 2019).
- Measurement of Free Iodine in Different Formulations of Povidone-Iodine Eye Drops 5.: This investigation uses phosphate-citrate buffer to measure the stability and release of iodine in ophthalmic solutions, highlighting its importance in ensuring the efficacy and safety of eye care products (Prado et al., 2019).
重悬
在25℃下，一片溶于100 mL去离子水中可得到0.05 M磷酸盐-柠檬酸盐缓冲液，pH 5.0。室温储存。
闪点(°F)
Not applicable
闪点(°C)
Not applicable
In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy.
Maria Stella Moreira et al.
Journal of photochemistry and photobiology. B, Biology, 166, 180-186 (2016-12-09)
Antonino Tuttolomondo et al.
Oncotarget, 8(37), 61415-61424 (2017-10-06)
Anderson-Fabry disease (AFD) is an inborn lysosomal enzymopathy resulting from the deficient or absent activity of the lysosomal exogalactohydrolase, α-galactosidase A. This deficiency, results in the altered metabolism of glycosphingolipids which leads to their accumulation in lysosomes, thus to cellular
Rose, N., et al.
Manual of Clinical Laboratory Immunology, 106-106 (1986)
P R Garrido et al.
Journal of photochemistry and photobiology. B, Biology, 194, 149-157 (2019-04-08)
Photobiomodulation therapy (PBMT) and the cell sheet (CS) technology improve processes relevant to tissue regeneration. The aim of this study was to investigate the effects of different PBMT parameters on the architecture (histology), protein composition (Western blotting and immunohistochemistry) and
Giuseppe Mangialardi et al.
Diabetologia, 62(7), 1275-1290 (2019-04-20)
Previous studies have shown that diabetes mellitus destabilises the integrity of the microvasculature in different organs by damaging the interaction between pericytes and endothelial cells. In bone marrow, pericytes exert trophic functions on endothelial cells and haematopoietic cells through paracrine
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