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Merck
CN

C9999

Sigma-Aldrich

柠檬酸盐缓冲液,pH 6.0,10×,抗原修复液

for immunohistochemistry

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别名:
2-羟基丙烷-1,2,3-三羧酸,混合氢氧化钠(Na(OH))和2-羟基丙烷-1,2,3-三羧酸钠
UNSPSC代码:
12352200
NACRES:
NA.47

应用

柠檬酸盐缓冲液,pH 6.0,10×,抗原修复液,在应用抗体或DNA荧光原位杂交之前,已被用作福尔马林固定的石蜡包埋(FFPE)组织切片上的热诱导抗原修复液。在免疫组化(IHC)中,最常用的固定剂(如福尔马林)通过其内在交联掩盖组织抗原(细胞、膜和核)。这种掩盖导致 IHC(免疫组化)染色差或无染色。在FFPE组织切片上使用柠檬酸盐缓冲液(pH 6.0)或其他抗原修复液改善了抗体对组织抗原的可及性。

制备说明

将柠檬酸盐缓冲液,pH 6.0,10x,抗原修复液用水稀释 10 倍,以制备1x 工作溶液,例如用 90mL水稀释 10mL 10x 浓缩液。

储存及稳定性

将产品储存在2-8°C。不要冻存,以避免产生沉淀。

其他说明

有时缓冲液可能含有淡黄色。这是由防腐剂引起的,不会影响缓冲液的功能。
将制备的1′ 柠檬酸盐缓冲液,pH 6.0,工作溶液储存于2-8℃。

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