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安全信息

L7759

Sigma-Aldrich

花生的凝集素

peroxidase conjugate, lyophilized powder

别名:

PNA, 花生凝集素

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.54

生物来源

Arachis hypogaea (peanut)

质量水平

200

偶联物

peroxidase conjugate

形式

lyophilized powder

peroxidase activity

≥20 units/mg protein

技术

western blot: suitable

储存温度

−20°C

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相关类别

应用

通用 Western 印迹法方案：
  • 糖蛋白样本量：500 ng
  • 凝集素浓度：0.1 μg/mL

  1. 每个泳道加入500 ng糖蛋白样品
  2. 运行 4-20％Bis-Tris SDS 页面凝胶
  3. 将凝胶转移至 PVDF 膜上
  4. 用 RTA 缓冲液（R0278 Sigma）在室温下封闭膜 1 小时
  5. 在室温下用 RIPA 缓冲液将 HRP 凝集素以 0.1ug / ml 孵育 2 小时
  6. 用 25ml RIPA 缓冲液洗涤膜 5×5 分钟
  7. 使用化学发光底物（CPS1-120）进行检测

生化/生理作用

花生凝集素不会凝集正常的人类红细胞，但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。

单位定义

在 20°C、pH 值为 6.0 条件下，1 个单位的邻苯三酚在 20 s 内会形成 1 mg 红桔酚。

外形

含柠檬酸钠

制备说明

采用改进的已发表方法，通过过氧化物酶 (P8375) 制备，有利于低分子量偶联物。通过亲和层析缀合后再纯化。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein?  If so, what is the carbohydrate content?

    Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate.  Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure".  Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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