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Merck
CN

L6135

Sigma-Aldrich

花生的凝集素

biotin conjugate, lyophilized powder

别名:

PNA, 花生凝集素

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

偶联物

biotin conjugate

质量水平

表单

lyophilized powder

分子量

120 kDa

组成

Protein, ≥80% Lowry

标记范围

~3 mol biotin per mol protein

技术

blood typing: suitable
fractionation: suitable

溶解性

water: 1.00-1.10 mg/mL, clear to slightly hazy, colorless

储存温度

2-8°C

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一般描述

花生凝集素(PNA)是豆科植物花生(Arachis hypogaea)的一种凝集素或凝集素,在摄入花生后在人的外周静脉血液中发现。PNA凝集素对半乳糖基(β-1,3)N-乙酰半乳糖胺具有特异性。

应用

来自花生(花生)的凝集素已被用于:
  • 酶联微孔板法检测β-D-半乳糖-N-乙酰氨基葡萄糖
  • 在免疫组织化学双重染色中
  • 免疫组织化学双重染色用于检测生发中心
  • 酶联微孔板法检测β-D-半乳糖-N-乙酰氨基葡萄糖

生化/生理作用

花生(花生)凝集素作为Thomsen-Friedenreich抗原(TF)的抗原决定簇和正常结肠上皮中结肠直肠癌刺激细胞分裂的促分裂原。因此,它被用作肿瘤标志物。
花生凝集素不会凝集正常的人类红细胞,但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。

包装

包装尺寸基于蛋白质含量。

外形

含有柠檬酸钠

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein?  If so, what is the carbohydrate content?

    Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate.  Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure".  Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

In situ observation of germinal center cell apoptosis during a secondary immune response
Saitoh HA, et al.
Journal of Clinical and Experimental Hematopathology : JCEH, 46, 73-82 (2006)
Synthesis of glycopolymers and their multivalent recognitions with lectins
Ting SRS, et al.
Polym. Chem., 1, 1392-1412 (2010)
Inhibition of influenza viral neuraminidase activity by collectins
Tecle T, et al.
Archives of Virology, 1731-1742 (2007)
Peanut (Arachis hypogaea) lectin recognizes α−linked galactose, but not N-acetyl lactosamine in N-linked oligosaccharide terminals
Chacko BK and Appukuttan PS
International Journal of Biological Macromolecules, 28, 365-371 (2001)
Peanut lectin stimulates proliferation of colon cancer cells by interaction with glycosylated CD44v6 isoforms and consequential activation of c-Met and MAPK: functional implications for disease-associated glycosylation changes
Glycobiology, 16(7), 594-601 (2006)

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