一般描述
应用
我们的TMB底物使用方法：
- 我们的TMB底物以即用型过氧化物酶底物的形式提供，其中的TMB溶于弱酸性缓冲液中。与过氧化物酶反应之前，底物溶液应呈无色至浅蓝绿色。与过氧化物酶反应时，底物系统形成蓝色反应产物。
- 加入酸终止反应后，测量TMB底物在450 nm处的光强。
- 我们的TMB底物推荐用于ELISA（微孔）程序，且已应用在各种测定方案中，如作为辣根过氧化物酶抗体偶联物的底物，用于检测亨德拉病毒糖蛋白、十四糖、sIgA和嗜中性白细胞胞外阱(NETs)。
- 不建议采用3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统用于膜应用。由于TMB底物会产生可溶性反应产物，因此不建议用于组织化学或印迹分析。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
No hydrogen peroxide should be added to product T0440. The product, which contains a peroxidase substrate, already contains hydrogen peroxide. This solution is ready to use for ELISA assays.
What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.
Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or conjugates be diluted. Usage information is described on our data sheet.
What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?
Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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