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T0440

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

别名:

TMB

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选择尺寸

100 ML
¥1,895.48
1 L
¥11,489.23
5 X 1 L
¥43,085.82

¥1,895.48

国内现货，预计发货时间2025年4月24日详情

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选择尺寸

变更视图
100 ML
¥1,895.48
1 L
¥11,489.23
5 X 1 L
¥43,085.82

About This Item

UNSPSC代码:
12352204
NACRES:
NA.83

¥1,895.48

国内现货，预计发货时间2025年4月24日详情

获取大包装报价

产品名称

ELISA 用 3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物系统, peroxidase substrate

质量水平

300

表单

liquid

储存温度

2-8°C

相关类别

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1 of 4

此商品
T4444T4319T8665
ELISA 用 3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物系统 peroxidase substrate

T0440

ELISA 用 3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物系统

3,3′,5,5′-四甲基联苯胺液体底物，超灵敏，用于 ELISA ready to use solution

T4444

3,3′,5,5′-四甲基联苯胺液体底物，超灵敏，用于 ELISA

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA peroxidase substrate

T4319

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA

3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物体系 peroxidase substrate

T8665

3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物体系

form

liquid

form

aqueous solution

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

TMB底物是辣根过氧化物酶-HRP的显色底物。

应用

TMB底物可用于利用辣根过氧化物酶结合物进行的ELISA程序。TMB底物会形成可溶的蓝色反应产物，可通过370或655 nm处的吸光度测定。该试剂可与样品中的亚硝酸盐反应，形成紫色的偶氮产物，可在450 nm处测量其吸光度。该反应可利用酸终止，形成黄色反应产物，并可通过450 nm处的光强精确测定。

我们的TMB底物使用方法：
  • 我们的TMB底物以即用型过氧化物酶底物的形式提供，其中的TMB溶于弱酸性缓冲液中。与过氧化物酶反应之前，底物溶液应呈无色至浅蓝绿色。与过氧化物酶反应时，底物系统形成蓝色反应产物。
  • 加入酸终止反应后，测量TMB底物在450 nm处的光强。
  • 我们的TMB底物推荐用于ELISA（微孔）程序，且已应用在各种测定方案中，如作为辣根过氧化物酶抗体偶联物的底物，用于检测亨德拉病毒糖蛋白、十四糖、sIgA和嗜中性白细胞胞外阱(NETs)。
  • 不建议采用3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统用于膜应用。由于TMB底物会产生可溶性反应产物，因此不建议用于组织化学或印迹分析。
注意：TMB底物为光敏材料，应避免接触直射阳光或紫外线。

推荐

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000439739
    0000439739
    0000430203
    0000430203
    0000411204

    没有发现合适的版本？

    如果您需要特殊版本，可通过批号或批次号查找具体证书。

    搜索COA

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    在文件库中查找您最近购买产品的文档。

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?

      No hydrogen peroxide should be added to product T0440. The product, which contains  a peroxidase substrate,  already contains hydrogen peroxide.  This solution is ready to use for ELISA assays.

    4. What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?

      The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.

    5. Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?

      No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or  conjugates be diluted. Usage information  is described on our data sheet.

    6. What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA,  and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?

      Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.

    7. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    8. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    9. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

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    Langmuir : the ACS journal of surfaces and colloids, 33(38), 9988-9996 (2017-08-29)
    OprF has a central role in Pseudomonas aeruginosa virulence and thus provides a putative target for either vaccines or antibiotic cofactors that could overcome the bacterium's natural resistance to antibiotics. Here we describe a procedure to optimize the production of
    Spontaneous and tetracosactide-induced anti-ACTH antibodies in man
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    Clinical Endocrinology, 84(4), 489-495 (2016)
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    Diagnostics (Basel, Switzerland), 10(1) (2020-01-16)
    Abstract: Paper-based colorimetric biosensors combine the use of paper with colorimetric signal detection. However, they usually demonstrate lower sensitivities because a signal amplification procedure has not been used. Stopping the reaction of colorimetric signal generation is often used in lab-based
    Médea Padra et al.
    Virulence, 9(1), 898-918 (2018-04-12)
    Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach. In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT
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