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应用
通用蛋白质印迹方案：
- 糖蛋白样品规格：500ng
- 凝集素浓度： 0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转凝胶至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
生化/生理作用
荆豆凝集素具有抗H血型特异性。凝集素有两种类型： UEA I 对 L-岩藻糖具有亲和力，而 UEA II 对 N,N′-二乙酰壳二糖具有亲和力。最初认为UEA I摩尔量为170,000道尔顿。 后来的报道表明，UEA I可以在中性和碱性pH下形成聚集体，正确的摩尔量是68,000道尔顿。
包装
包装尺寸基于蛋白质含量。
联系
由亲和纯化的凝集素制备缀合物。
单位定义
一单位在20℃、pH 6.0条件下，在20秒内从邻苯三酚形成1.0 mg的红棓酚。
外形
含有柠檬酸钠缓冲盐
制备说明
由过氧化物酶，VI型（P8375），使用Avrameas和Ternyck的两步戊二醛方法制备。
偶联后通过亲和层析重新纯化。
偶联后通过亲和层析重新纯化。
分析说明
凝集活性以μg/ml表示，由磷酸盐缓冲盐水（pH 6.8）连续稀释1 mg/ml溶液而测得。该活性是指25℃孵育1小时后使2％人O型血红细胞悬浮液凝集的最低浓度。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
常规特殊物品
Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
S Avrameas et al.
Immunochemistry, 8(12), 1175-1179 (1971-12-01)
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