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安全信息

H1136

Sigma-Aldrich

透明质酸酶 来源于 Streptomyces hyalurolyticus

别名:

透明质酸裂解酶 来源于 Streptomyces hyalurolyticus

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About This Item

CAS号:
37259-53-3
EC 号:
4.2.2.1 (BRENDA, IUBMB)
EC 号:
253-430-8
MDL编号:
MFCD00131347
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

Streptomyces sp. (S. hyalurolyticus)

质量水平

200

偶联物

conjugate (Glucosaminoglycan)

形式

film (Clear)

包装

ampule of 300 ×  units

储存温度

−20°C

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相关类别

一般描述

透明质酸酶广泛存在于自然界。在人体中，它存在于不少器官和体液中。透明质酸酶见于病毒、噬菌体、真菌和细菌的分泌物。它还存在于线虫和蚂蟥的分泌物中。蛇和蝎子的毒液中会产生透明质酸酶。蜜蜂、蜘蛛、黄蜂、大黄蜂、毛虫、鱼类和蜥蜴也会分泌透明质酸酶。

应用

透明质酸酶（来自Streptomyces hyalurolyticus）已用于：
  • 制备DMEM/F-12（杜氏改良Eagle培养基/营养混合物F-12）培养基，用于从分离肿瘤组织中分离和纯化单细胞
  • 作为消化液组分，用于从睾丸组织获得生殖干细胞

生化/生理作用

透明质酸裂解酶在β-D-GlcNAc-(1→4)-β-D-GlcA键上切割透明质酸，得到3-(4-脱氧β-D-葡萄糖-4-烯基糖基)-N-乙酰基-D-葡糖胺四糖和六糖。与其他透明质酸酶不同，该酶对透明质酸具有特异性，并且对软骨素和硫酸软骨素无活性。
透明质酸酶属于内切糖苷酶，通过配合β-葡糖醛酸酶和β-N-乙酰氨基葡萄糖苷酶等外切糖苷酶完成其内切功能。透明质酸酶调节透明质酸的周转代谢。

单位定义

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

其他说明

有关复合碳水化合物分的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product H1136, Hyaluronidase, be reconstituted?

    Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.

  4. What amount of product is present in the vial of Product H1136, Hyaluronidase?

    The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.

  5. What is the molecular weight of Product H1136, Hyaluronidase?

    The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000 DALTONS as determined by gel filtration.

  6. Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?

    No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.

  7. How is the activity of Product H1136, Hyaluronidase,determined?

    Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  11. I can’t see any material in the container. Is material present in the container?

    The product is lyophilized directly into the ampule and its appearance is a clear, thin film.

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实验方案

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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