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Merck
CN

H3506

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type I-S, lyophilized powder, 400-1000 units/mg solid

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别名:
玻璃糖醛酸酶, 玻璃酸酶
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

类型

Type I-S

质量水平

形式

lyophilized powder

比活

400-1000 units/mg solid

分子量

~55 kDa (four subunits of 14 kDa each)

技术

cell culture | mammalian: suitable
single cell analysis: suitable

UniProt登记号

应用

diagnostic assay manufacturing

运输

wet ice

储存温度

−20°C

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应用

来自牛睾丸的透明质酸酶已用于乳腺组织、心脏组织和卵母细胞的消化以用于细胞的分离。

适用于制备用于测序的单细胞悬液。

生化/生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸,并已发现可在癌症进展期间会被不适当地调节。

单位定义

在 37 ºC、pH 5.35 条件下,一单位每分钟可降解 0.75 μg 多糖透明质酸(通过比浊吸光度测量(λ: 600nm),与 BSA 复合 45 分钟后测量)。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. How is Hyaluronidase from bovine testes, Product H3506, solubilized?

    It is soluble at 1 mg/ml in 0.02 M phosphate buffer, pH 7 containing 77 mM sodium chloride and 0.01% bovine serum albumin.

  3. How is a solution of Hyaluronidase from bovine testes, Product H3506, stored?

    Prepare fresh solutions directly before use since stock solutions are not stable for storage.

  4. Can Hyaluronidase from bovine testes, Product H3506, be used on humans?

    No. None of our packaged research products are for human use. 

  5. What is the shelf life of Product H3506, Hyaluronidase from bovine testes?

    Product H3506, Hyaluronidase from bovine testes, has a Recommended Retest period of two years (period of time between the Quality Control Release and Recommended Retest dates).  This date assigns the time frame during which the batch is expected to remain within established specifications if stored under Sigma-Aldrich’s defined conditions, which for product H3506 is at -20°C.  This information is provided on the Product's lot specific Certificate of Analysis. 

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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