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主要文件

安全信息

H0267

Sigma-Aldrich

亚铁稳定化血红蛋白 A0 人

lyophilized powder

别名:

Hemoglobin A0 Ferrous Stabilized, Hemoglobin-A0

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About This Item

CAS号:
54651-57-9
EC 号:
259-278-9
MDL编号:
MFCD00131287
UNSPSC代码:
12352202
eCl@ss:
42030116
NACRES:
NA.61

生物来源

human

质量水平

200

方案

97-100% (agarose gel electrophoresis)

表单

lyophilized powder

技术

immunofluorescence: suitable

溶解性

H2O: soluble 20 mg/mL

适用性

suitable for electrophoresis and chromatography standard

UniProt登记号

P68871

储存温度

−20°C

基因信息

human ... HBB(3043)

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一般描述

血红蛋白是红细胞的主要成分，也是红细胞呈现红色的原因。它在红细胞中的正常浓度为34％。由于它能够将氧从肺部输送到身体组织，促进二氧化碳的返回转运，因此血红蛋白是脊椎动物最重要的呼吸蛋白。
has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).

应用

亚铁稳定化人血红蛋白A0可用于：
  • 作为非荧光吸收剂制备仿组织模型
  • 了解冠状动脉斑块内出血如何引发新型抗动脉粥样硬化巨噬细胞表型
  • 研究血红蛋白（Hb）A0、HbA2和HbS对HbF测定的干扰

血红蛋白A0用于了解冠状动脉斑块内出血如何引发新型抗动脉粥样硬化巨噬细胞表型。它可用于时间分辨免疫荧光法测定胎儿血红蛋白。

生化/生理作用

血红蛋白（Hb）编码基因的变异会导致血红蛋白病（如地中海贫血）和非典型结构变异（如Hb Lepore和HbE）。

包装

包装规格标示出通过 Drabkin, D.L., J. Biol. Chem., 164, 703 (1946) 中介绍的方法测定的血红蛋白含量。

重悬

用缓冲液复原时，可得到 >85% 的亚铁血红蛋白。

免责声明

供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000383203
0000371900
0000360025
0000323542
0000323543

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the structure of Product H0267, Hemoglobin A0?

    Hemoglobin A0 is the major hemoglobin protein of adult hemoglobin. It is a tetrameric protein consisting of 2 alpha and 2 beta chains, each of which contains a heme moiety and iron.

  4. What is the molecular weight of H0267 Hemoglobin A0?

    Sigma has not evaluated the molecular weight of hemoglobin.  Based on the reported sequence and including the weight of the 4 heme groups and 4 iron atoms, the molecular weight is approximately 64,677 Daltons. The Structure and Action of Proteins, p. 52, Harper and Row (1969).

  5. How can Product H0267, Hemoglobin A0, be dissolved?

    Hemoglobin is soluble in water at 20 mg/mL.  Solutions in phosphate buffer at about neutral pH can also be prepared.

  6. What is the solution stability of Product H0267, Hemoglobin A0?

    Solutions of ferrous hemoglobin are not stable indefinitely due to the likely autoxidation of the heme group leading to the formation of the ferric derivative (methemoglobin). It is recommended to prepare solutions fresh in deaerated water or buffer.  If necessary, store such prepared solutions no more than 1-2 days at 2-8°C.

  7. Is there methemoglobin in Product H0267, Hemoglobin A0?

    The product may contain a small amount of methemoglobin as described on the specification sheet.

  8. Can Product H0267, Hemoglobin A0, be used in functional studies involving the binding and release of oxygen as a ligand?

    Sigma has not validated this hemoglobin for use in functional studies. The iron in the heme moiety is in the +2 state.  However, this does not mean that this hemoglobin will behave necessarily like native hemoglobin nor be useful for functional studies of the molecule.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Motasam Majedy et al.
Journal of biomedical optics, 27(7) (2021-12-02)
For optical methods to accurately assess hemoglobin oxygen saturation in vivo, an independently verifiable tissue-like standard is required for validation. For this purpose, we propose three hemoglobin preparations and evaluate methods to characterize them. To spectrally characterize three different hemoglobin
Randi E Gislefoss et al.
Biopreservation and biobanking, 19(6), 525-530 (2021-10-07)
Background: During sampling and processing, blood samples can be affected by hemolysis. Information is lacking regarding hemolysis for biobank samples. There is a need for a method that can easily measure hemoglobin as an indicator of hemolysis in stored samples
Seung Yup Lee et al.
Science advances, 6(47) (2020-11-22)
Pancreatic cancer is one of the deadliest cancers, with a 5-year survival rate of <10%. The current approach to confirming a tissue diagnosis, endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), requires a time-consuming, qualitative cytology analysis and may be limited because of
Screening and diagnosis for haemoglobin disorders
Prevention of Thalassaemias and Other Haemoglobin Disorders, 1 (2013)
Vivide Tuan-Chyan Chang et al.
Neoplasia (New York, N.Y.), 11(4), 325-332 (2009-03-25)
Cervical cancer is the second most common female cancer worldwide. The ability to quantify physiological and morphological changes in the cervix is not only useful in the diagnosis of cervical precancers but also important in aiding the design of cost-effective
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