生物来源
bovine blood
质量水平
形式
substrate powder
分子量
Mr ~64500
技术
activity assay: suitable
溶解性
H2O: soluble 20 mg/mL
适用性
suitable for protease substrate
储存温度
2-8°C
基因信息
cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)
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一般描述
Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.
Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.
应用
Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.
α-弹性蛋白的溶解度已被应用于弹性蛋白模拟生物材料的构建。
生化/生理作用
氧气转运蛋白。Fe2+/Fe3+平衡是血液氧合的一种生理指标；脱氧血红蛋白通过将亚硝酸还原为NO来补充反馈回路，而NO是一种可增强血液流向缺氧组织流量的血管扩张剂。
Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.
注意
由于天然血红蛋白在空气中易于氧化，因此这些制剂主要为高铁血红蛋白。
制备说明
Prepared from washed, lysed and dialyzed erythrocytes.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
Luke J Norbury et al.
Biochimie, 93(3), 604-611 (2010-12-21)
Cathepsin proteases are promising vaccine or drug targets for prophylaxis or therapy against Fasciola parasites which express cathepsin L and B proteases during their development. These proteases are believed to be involved in important functions for the parasite, including excystment
A study of the interaction of bovine hemoglobin with synthetic dyes using spectroscopic techniques and molecular docking
Bansal S, et al.
Frontiers in Chemistry, 4(4), 50-50 (2017)
High pressure effects on the activities of cathepsins B and D of mackerel and horse mackerel muscle
Fidalgo L, et al.
Czech Journal of Food Sciences, 32(2), 188-193 (2016)
Xianbo Lu et al.
Biosensors & bioelectronics, 23(8), 1236-1243 (2007-12-18)
Carbon nanofibers (CNFs), with typical diameters of approximately 80 nm and lengths of the order of micrometers, are extremely attractive in bioanalytical area as they can combine properties of high surface area, non-toxicity, acceptable biocompatibility, ease of fabrication, chemical and
Characteristics of bovine hemoglobin as a potential source of hemoglobin-vesicles for an artificial oxygen carrier
Sakai H, et al.
Journal of Biochemistry, 131(4), 611-617 (2002)
实验方案
本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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