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Merck
CN

D5941

Sigma-Aldrich

Drabkin试剂

for the determination of hemoglobin

别名:

Hemoglobin Quantitation Reagent

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About This Item

UNSPSC代码:
12352402
NACRES:
NA.25

质量水平

表单

liquid

一般描述

Drabkin′s 试剂用于测量血液样本中的血红蛋白。它的组份包括铁氰化钾、氰化钾和磷酸二氢钾。铁氰化钾将血红蛋白氧化为高铁血红蛋白,然后氧化为氰化高铁血红蛋白。氰化高铁血红蛋白水平估计为 530nm。这是一种可靠的方法,可以测量除磺胺血红蛋白以外的所有血红蛋白形式。

应用

Drabkin′s 试剂已用于定量比色法测定脑组织中的血红蛋白,以评估脑出血、在红细胞中检测溶血以及对侧和同侧脑组织匀浆。

组分

包含碳酸氢钠、铁氰化钾和氰化钾。

警示用语:

Danger

危险分类

Acute Tox. 2 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - STOT RE 2

靶器官

Thyroid

补充剂危害

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

法规信息

监管及禁止进口产品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. Can Product D5941, Drabkin's reagent, be used to determine the Hemoglobin in matrigel plugs?

    This reagent cannot used for the determination of Hemoglobin in Matrigel plugs. This is because the sample being tested must be soluble so that it can be read in a spectrophotometer.

  2. How do I dissolve Product D5941, Drabkin's reagent, for use?

    Reconstitute one vial of Drabkin's Reagent with 1000 ml of water. Then add 0.5 ml of the 30% Brij 35 Solution, Product Code B4184, to the 1000 ml of reconstituted Drabkin’s Reagent. Mix well and filter if insoluble particles remain.

  3. I do not understand Hemoglobin concentrations in the standard curve table in the Datasheet for Product D5941, Drabkin's reagent.

    The Hemoglobin standard concentrations of 60, 120, and 180 mg/mL listed in Column 4 of the table in the datasheet are corrected by the dilution factor of 1:251 (which is the dilution factor for 40 microliters of Hemoglobin Standard diluted in 10 mL of Drabkins's Reagent). This is done so that the user does not need to remember to multiply all calculated Hemoglobin concentrations in the unknowns being assayed by 251. The datasheet describes the preparation of a 180 mg/mL Cyanmethemoglobin Standard Solution. If you do not have an appropriate 180 mg/mL Cyanmethemoglobin Standard, prepare a 0.7171 mg/ml Cyanmethemoglobin Standard Solution by dissolving a suitable Hemoglobin in Drabkins's reagent. (c.f. Product No. H7379). Take any water content into account in weighing the Hemoglobin. Do not dilute this standard as described in step (2) of the datasheet.

  4. What should I use as a Cyanmethemoglobin Standard Solution for Product D5941, Drabkin's reagent?

    Product No. H7379 Hemoglobin human can be used by dissolving in Drabkin's solution at a concentration of 0.7171 mg/ml. This will be equivalent to a 180 mg/ml Cyanmethemoglobin Standard Solution that is diluted 1:251 per our Datasheet. It is not necessary to use a Hemoglobin standard from the same species as is being tested.

  5. Is Product D5941, Drabkin's reagent, the same as the Diagnostic reagent 525-2 or Diagnostic kit 525A?

    This reagent shares the same formulation as the discontinued Sigma Diagnostics product 525-2 but it is intended only for research use, not for In Vitro Diagnostic use. The Diagnostic kit included a Hemoglobin standard that is no longer sold.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods.
Balasubramaniam P and Malathi A
Journal of Postgraduate Medicine, 38(1), 8-8 (1992)
Drabkin, D.L., Austin, J.H.
The Journal of Biological Chemistry, 112, 51-51 (1935)
Toxic effects of silver nanoparticles and nanowires on erythrocyte rheology
Kim MJ and Shin S
Food And Chemical Toxicology, 67(6), 80-86 (2014)
Kang Nian Yap et al.
The Journal of experimental biology, 220(Pt 22), 4282-4291 (2017-09-28)
Foraging to obtain food, either for self-maintenance or at presumably elevated rates to provide for offspring, is thought to be an energetically demanding activity but one that is essential for fitness (higher reproductive success and survival). Nevertheless, the physiological mechanisms
Non-invasive multimodality imaging directly shows TRPM4 inhibition ameliorates stroke reperfusion injury
Chen B, et al.
Translational Stroke Research, 1-13 (2018)

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