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一般描述
应用
特点和优势
- 该试剂为即用型。 无需混合或稀释。
- 显色很快速。 仅需五分钟的孵育便能将样本在595 nm处进行读值。
- 带有硫醇的还原性糖和还原性底物并不会干扰该试剂。
- 试剂适用于微量(1-10 μg/ml)及标准(50-1400 μg/ml)的检测。
- 可用于微孔板检测。
- 低成本检测。
法律信息
应用
警示用语：
Warning
危险分类
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
靶器官
Eyes,Central nervous system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?
A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.
What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?
The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.
When using Product B6916, Bradford Reagent, how soon after my color development do I need to read my assay?
he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.
When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?
The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).
Is the BSA standard supplied with Product B6916, Bradford Reagent?
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
实验方案
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
相关内容
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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