Skip to Content

很抱歉，目前系统维护中。在此期间，网站下单功能无法使用。给您带来不便，深表歉意。如需其他帮助，请联系客户联络中心：400 620 3333。

Merck
CN
All Photos(4)

Key Documents

View All Documentation

Safety Information

DUO92013

Sigma-Aldrich

Duolink® In Situ Detection Reagents FarRed

Synonym(s):

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
Product DUO92013 is not currently sold in your country. Contact Technical Service

product line

Duolink®

technique(s)

proximity ligation assay: suitable

fluorescence

λex 644 nm; λem 669 nm (Cyanine 5, Zeiss Filter set 50)

suitability

suitable for fluorescence

shipped in

dry ice

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
497283067442424
Sigma-Aldrich

92716

Atto 620

Sigma-Aldrich

49728

Atto 620 maleimide

Atto 700 BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

30674

Atto 700

Sigma-Aldrich

42424

Atto 550

fluorescence

λex 617 nm; λem 641 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 619 nm; λem 643 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

fluorescence

-

form

powder

form

solid

form

-

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

≥90% (HPCE)

assay

≥80% (coupling to thiols)

assay

≥90.0% (HPCE)

assay

≥90% (HPLC)

product line

BioReagent

product line

-

product line

BioReagent

product line

-

General description

Duolink® In Situ Detection Reagents Far Red contains all the necessary Duolink In situ reagents to perform the amplification and detection of bound PLA® probes. The detection probes contain a fluorophore (lex = 644nm and lem = 669nm), which may be visualized using the same filter as Cy®5. Experiments conducted using Duolink In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.[1][2][3]

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
FarRed fluorescence detection reagents are often used with Cyanine 5 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Components

  • 5x Ligation - Contains oligonucleotides that hybridize to the PLA probes and all components needed for ligation except the Ligase
  • 1x Ligase (1 unit/μL)
  • 1x Polymerase (10 units/μL)
  • 5x Amplification FarRed - Contains all components needed for Rolling Circle Amplification (RCA) except the Polymerase. It also contains oligonucleotide probes labeled with a fluorophore that hybridize to the RCA product.
See datasheet for more information.

Not included in Detection kit:

Primary antibodies, PLA probes, wash buffers, mounting medium

Preparation Note

Storage and Stability: Store the components at –20 °C. The enzymes should be kept cold (–20 °C) at all times, use a freezing block when removing them from the freezer.

Legal Information

Cy is a registered trademark of Cytiva
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Regulatory Information

常规特殊物品
含少量动物源组分生物产品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ7936
    SLCQ4798
    SLCQ4802
    SLCN5770
    SLCM6160

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Profiling of protein?protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors.
    Lee H W, et al.
    Nature Biomedical Engineering, 2(4), 239-239 (2018)
    Oliver Werz et al.
    Nature communications, 9(1), 59-59 (2018-01-06)
    Proinflammatory eicosanoids (prostaglandins and leukotrienes) and specialized pro-resolving mediators (SPM) are temporally regulated during infections. Here we show that human macrophage phenotypes biosynthesize unique lipid mediator signatures when exposed to pathogenic bacteria. E. coli and S. aureus each stimulate predominantly
    M Aubele et al.
    British journal of cancer, 103(5), 663-667 (2010-08-12)
    Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression
    Kan V Lu et al.
    Cancer cell, 22(1), 21-35 (2012-07-14)
    Inhibition of VEGF signaling leads to a proinvasive phenotype in mouse models of glioblastoma multiforme (GBM) and in a subset of GBM patients treated with bevacizumab. Here, we demonstrate that vascular endothelial growth factor (VEGF) directly and negatively regulates tumor
    Pengcheng Zhu et al.
    Cancer cell, 19(3), 401-415 (2011-03-15)
    Cancer is a leading cause of death worldwide. Tumor cells exploit various signaling pathways to promote their growth and metastasis. To our knowledge, the role of angiopoietin-like 4 protein (ANGPTL4) in cancer remains undefined. Here, we found that elevated ANGPTL4
    View All Related Papers

    Articles

    How Proximity Ligation Assays (PLA) Work

    Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.

    How to Optimize the Duolink® Proximity Ligation Assay

    Things to consider for preparation, setup and execution of the Duolink® assay protocol

    Duolink® PLA Troubleshooting Guide

    Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

    Protocols

    Duolink® PLA Fluorescence Protocol

    This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

    Duolink® In Situ Short Instructions - Fluorescence

    This page details the Duolink® In Situ Short Protocol for fluorescence detection

    Related Content

    Duolink® PLA Applications

    Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service