Recommended Products
grade
for fluorescence
Quality Level
Assay
≥90% (HPLC)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 553-559 nm Amax
storage temp.
−20°C
General description
Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3™ and is characterized by a high photostability.
find more information here
find more information here
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3™, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Cy3 is a trademark of Cytiva
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Sandeep Kumar Vashist
Analytical biochemistry, 421(1), 336-338 (2011-11-19)
A surface plasmon resonance (SPR)-based procedure was developed to determine the effect of antibody modifications on its biomolecular binding behavior. Mouse immunoglobulin G (IgG) was immobilized on a protein A-functionalized gold-coated SPR chip. Goat anti-mouse IgG and its various commercially
Rula Masoud et al.
ACS nano, 6(7), 6272-6283 (2012-06-06)
We present a test case example of a detailed single-molecule fluorescence study of one of the most sophisticated and complex DNA devices introduced to date, a recently published autonomous bipedal DNA motor. We used the diffusion-based single-molecule Förster resonance energy
Rahul Roy et al.
Nature methods, 5(6), 507-516 (2008-05-31)
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by
Articles
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service