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BCA1

Sigma-Aldrich

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

Synonym(s):

BCA protein assay

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250 G
CN¥1,000.26
1 KG
CN¥4,616.55

CN¥1,000.26

List priceCN¥1,445.76Save 31%

Available to ship onApril 09, 2025Details

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250 G
CN¥1,000.26
1 KG
CN¥4,616.55

About This Item

UNSPSC Code:
12352106
NACRES:
NA.32

CN¥1,000.26

List priceCN¥1,445.76Save 31%

Available to ship onApril 09, 2025Details

usage

sufficient for ~500 assays

storage temp.

2-8°C

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甲醇 gradient grade for liquid chromatography LiChrosolv® Reag. Ph Eur

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Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

assay

≥99.9% (GC)

assay

≥99.8% (GC)

assay

≥99.8% (GC)

assay

-

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

form

liquid

form

liquid

form

liquid

form

-

vapor pressure

128 hPa ( 20 °C), 97.68 mmHg ( 20 °C), 410 mmHg ( 50 °C)

vapor pressure

128 hPa ( 20 °C), 410 mmHg ( 50 °C), 97.68 mmHg ( 20 °C)

vapor pressure

128 hPa ( 20 °C), 410 mmHg ( 50 °C), 97.68 mmHg ( 20 °C)

vapor pressure

410 mmHg ( 50 °C), 97.68 mmHg ( 20 °C)

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples,[1] human embryonic kidney 293 cells[2] and luteal cells.[3]

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H290,H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

含少量动物源组分生物产品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000422951
    0000422951
    0000412486
    0000412486
    0000389196

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    Find documentation for the products that you have recently purchased in the Document Library.

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    D Jantas et al.
    Neurotoxicity research, 25(2), 208-225 (2013-10-10)
    Tianeptine (Tian) possesses neuroprotective potential, however, little is known about the effect of this drug in models of neuronal apoptosis. In the present study, we aimed (1) to compare the neuroprotective capacities of some antidepressants (ADs) in the models of
    TGFB1 modulates in vitro secretory activity and viability of equine luteal cells
    Galvao A, et al.
    Cytokine, 110, 316-327 (2018)
    Human OATP1B1 (SLCO1B1) transports sulfated bile acids and bile salts with particular efficiency
    Toth B, et al.
    Toxicology in vitro, 52, 189-194 (2018)
    Salivary proteome patterns of individuals exposed to High Altitude
    Jain S, et al.
    Archives of Oral Biology, 96, 104-112 (2018)
    R E Brown et al.
    Analytical biochemistry, 180(1), 136-139 (1989-07-01)
    Protein quantitation based on bicinchoninic acid (BCA) is simple, sensitive, and tolerant to many detergents and substances known to interfere with the Lowry method. However, certain compounds often used during protein purification do interfere with the BCA protein assay. The
    View All Related Papers

    Articles

    Determination of Protein by Bicinchoninic Acid Assay

    BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

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    Tools for Protein Quantitation

    Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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