Skip to Content
Merck
CN
All Photos(5)

Documents

Safety Information

A2153

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

Sign Into View Organizational & Contract Pricing
All Photos(5)

About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

200

Assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

impurities

≤6.0% Loss on Drying

loss

≤6%

pH

6.5-7.5 (1% in 0.15 M sodium chloride)
7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Bovine Serum Albumin (BSA) is a globular, non-glycosylated α-helical plasma protein produced in the bovine liver. It consists of three domains (I, II, and III), each with two sub-domains (A and B), arranged in a heart-shaped structure.

Application

Bovine serum albumin has been used-

  • along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
  • as a component of modified Krebs-Ringer buffer (dmKRBT)
  • in gel shift analysis
  • as a blocking agent in immunocytochemistry, immunohistochemistry,(4) immunofluorescence protocol and sandwich enzyme-linked immunosorbent assay (ELISA)
  • as a component of phosphate buffer saline (PBS) solution to resuspend cells for analysis during flow cytometry
  • as a protein standard to quantify proteins in tissue samples via Bradford’s method
  • to measure the enzyme activity of 17β HSD enzyme

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Is Product A2153, Albumin from bovine serum (BSA), suitable for use in ELISA?

    We have not tested A2153 in quality control for use in ELISA applications. Because it contains globulins, we would generally not recommend A2153 for use in ELISA.

  2. Is Product A2153, Albumin from bovine serum (BSA), suitable for use in immunoblotting experiments as a blocking agent?

    We have not tested A2153 in quality control for use in immunoblotting applications as a blocking agent. Because it contains globulins, we would generally not recommend A2153 for use as a blocking agent.

  3. Product A2153, Albumin from bovine serum (BSA), powder looks tan in color.  Is this OK?

    The tan color is within specifications. The color can vary because of factors such as concentration of a solution at the time of lyophilization.

  4. Is Product A2153, Albumin from bovine serum (BSA), suitable for cell culture?

    We do not test A2153 for cell culture use, or for any specifications such as endotoxin content. Customers will have to exercise their own judgment as to whether they wish to use A2153 in cell culture, or can search the literature for publications that cite this product number.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Simple histochemical stain for acrosomes on sperm from several species.
Larson JL and Miller DJ
Molecular Reproduction and Development, 52(4), 445-449 (1999)
Bartolomeo Della Ventura et al.
PloS one, 12(2), e0171754-e0171754 (2017-02-10)
Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue
Mateusz Majda et al.
Developmental cell, 43(3), 290-304 (2017-11-08)
The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result
D Gholobova et al.
Scientific reports, 8(1), 12206-12206 (2018-08-17)
The development of laboratory-grown tissues, referred to as organoids, bio-artificial tissue or tissue-engineered constructs, is clearly expanding. We describe for the first time how engineered human muscles can be applied as a pre- or non-clinical model for intramuscular drug injection
K Elaine Ritter et al.
Frontiers in neuroscience, 11, 690-690 (2018-01-10)
The autonomic and sensory nervous systems are required for proper function of all visceral organs, including the lower urinary tract (LUT). Despite the wide prevalence of bladder dysfunction, effective treatment options remain limited. Pelvic innervation regenerative strategies are promising, but
View All Related Papers

Related Content

Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service