D1315
DT透明质酸酶（NQO1） 人
lyophilized powder, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
别名:
DTD, NQO1, 醌还原酶
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关于此项目
Specific activity:
≥100 units/mg protein
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
产品名称
DT透明质酸酶（NQO1） 人, lyophilized powder, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
≥100 units/mg protein
mol wt
monomer 31000
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
human ... NQO1(1728)
Application
人双硫腙酶已用于研究评估新型醌磷二酰胺前药的开发。人DT黄递酶也被用于研究其晶体结构，以开发其与细胞毒性前体药物5-（氮丙啶-1-基）-2，4-二硝基苯甲酰胺（CB1954）相互作用的模型。
Biochem/physiol Actions
NQO1是一种胞质同型二聚体FAD依赖酶，催化大量细胞毒性醌类减少，从而保护细胞免受氧化应激。此外，氧化应激也可能增强NQO1介导的p53和p73对蛋白酶体降解的保护。NQO1的高诱导表达受Nrf2-Keap1/ARE通路控制，似乎受氧化应激敏感性变化的影响。在缺氧无糖期间，NQO1似乎参与了AMPK诱导的癌细胞死亡。 NQO1在多种实体瘤（包括乳腺癌、胰腺癌、肺癌和结肠癌等）中均有过度表达。
双硫腙酶，也称为NAD（P）H：（醌受体）氧化还原酶，参与几种细胞毒性抗肿瘤醌类和硝基苯类的还原活化过程。它使用NADH或NADPH作为电子供体，催化醌类和醌类化合物的双电子还原成氢醌类。黄素酶每摩尔酶含有1摩尔FAD。
显示体内激活醌基抗肿瘤剂。适合与载体分子结合。
Other Notes
一个单位将在37℃甲萘醌底物存在下每min/mg还原1.0 & # 956；摩尔细胞色素C
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
S Chen et al.
The Journal of biological chemistry, 272(3), 1437-1439 (1997-01-17)
DT-diaphorase (EC 1.6.99.2), also referred to as NAD(P)H:(quinone-acceptor) oxidoreductase, is involved in the reductive activation process of several cytotoxic antitumor quinones and nitrobenzenes. It has been observed in our and other laboratories that the rat enzyme is significantly more effective
David Siegel et al.
Frontiers in pharmacology, 13, 1015642-1015642 (2022-11-22)
The stress induced protein NQO1 can participate in a wide range of biological pathways which are dependent upon the interaction of NQO1 with protein targets. Many of the protein-protein interactions involving NQO1 have been shown to be regulated by the
Yang Yang et al.
Journal of experimental & clinical cancer research : CR, 33, 14-14 (2014-02-07)
NAD (P) H: quinone oxidoreductase 1 (NQO1) is a xenobiotic metabolizing enzyme that detoxifies chemical stressors and antioxidants, providing cytoprotection in normal tissues. However, high-level expression of NQO1 has been correlated with numerous human malignancies, suggesting a role in carcinogenesis
C Flader et al.
Journal of medicinal chemistry, 43(16), 3157-3167 (2000-08-24)
A series of naphthoquinone and benzimidazolequinone phosphorodiamidates has been synthesized and studied as potential cytotoxic prodrugs activated by DT-diaphorase. Reduction of the quinone moiety in the target compounds was expected to provide a pathway for expulsion of the phosphoramide mustard
Molecular of Modelling of Human DT-Diaphorase for Enzyme-Directed Bioreductive Drug Design.
Doughtyl, S.W. and Phillips, R.M.
Molecular Simulations, 24, 209-209 (2000)
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