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重组
expressed in E. coli
质量水平
检测方案
≥90% (SDS-PAGE)
形式
lyophilized powder
比活
≥100 units/mL
分子量
monomer 24000
环保替代产品特性
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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sustainability
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环保替代产品分类
运输
wet ice
储存温度
−20°C
基因信息
Escherichia coli K12 ... nfsB(945483)
相关类别
一般描述
硝基还原酶是一种黄素蛋白，由NfsB基因编码。它包含一个二聚体，每个亚基具有217个氨基酸和活性位点。该结构具有FMN和与酶结合的底物。
应用
大肠杆菌来源硝基还原酶可结合猪肝酯酶(PLE)用于偶联结合反应。它还还可结合探针HyCL-3和HyCL-4-AM针对大鼠肝微粒体进行化学发光响应研究。
生化/生理作用
硝基还原酶(NTR)可催化还原硝基芳香族底物和醌。NTR的F124K突变体可用于癌症治疗，改善药物CB1954的敏化作用。
硝基还原酶在NADPH或NADH为还原剂的氧还系统中有重要作用。
硝基还原酶增强了生命体对于含氮药物，比如甲硝唑的敏感度，它将氮基团转化为有毒的氮自由基。
能够还原奎宁。 该酶可以激活抗体引导酶前药治疗的前药。
物理属性
含有PBS的冻干粉。 作为辅药不含有BSA
单位定义
在pH7.4、37℃、存在甲萘醌和NADH的条件下，一单位酶每分钟能够还原1μmole细胞色素C。
制备说明
使用没有动物组分的材料生产。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Sébastien Jenni et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 248, 119179-119179 (2020-11-30)
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical
G M Anlezark et al.
Biochemical pharmacology, 50(5), 609-618 (1995-08-25)
A nitroreductase isolated and purified from Escherichia coli B has been demonstrated to have potential applications in ADEPT (antibody-directed enzyme prodrug therapy) by its ability in vitro to reduce dinitrobenzamides (e.g. 5-aziridinyl 2,4-dinitrobenzamide, CB 1954 and its bischloroethylamino analogue, SN
Mansooreh Jaberipour et al.
Biochemical pharmacology, 79(2), 102-111 (2009-08-12)
Prodrug activation gene therapy for cancer involves expressing prodrug-activating enzymes in tumour cells, so they can be selectively killed by systemically administered prodrug. For example, Escherichia colinfsB nitroreductase (E.C. 1.6.99.7)(NTR), sensitises cells to the prodrug CB1954 (5-[aziridin-1-yl]-2,4-dinitrobenzamide), which it converts
Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954
Grove JI, et al.
Cancer research, 63(17), 5532-5537 (2003)
Mckayla Stevens et al.
Bioorganic & medicinal chemistry, 28(22), 115710-115710 (2020-10-03)
In two previous studies, we identified compound 1 as a moderate GroEL/ES inhibitor with weak to moderate antibacterial activity against Gram-positive and Gram-negative bacteria including Bacillus subtilis, methicillin-resistant Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, and SM101 Escherichia coli (which has
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