一般描述
Research area: Cancer
应用
克鲁维氏梭菌的心肌黄酶已用于鳃细胞中的乳酸脱氢酶测定，并浸渍在条带上进行甲酸脱氢酶测定。
生化/生理作用
心肌黄酶在电子受体（例如亚甲基蓝或2，6-二氯苯酚吲哚酚）存在下催化β-NADH或β-NADPH的氧化。 已知对β-NADH或β-NADPH具有特异性的心肌黄酶。Straub的猪心脏酶可能具有天然心肌黄酶（β-NADH特异性）以及硫辛酸和硫辛酰胺脱氢酶活性。据报道它是一种单体蛋白质。然而，Massey报道心肌黄酶可能是一种变性的脂硫辛酰胺脱氢酶。将猪心脏制剂与Cu2+进行预孵育，可降低硫辛酰胺脱氢酶活性并呈比例增加β-NADH心肌黄酶活性。在我们的实验室中，我们已经在一定程度上证明了这种对猪心脏酶的铜效应，但在克氏梭菌 或圆酵母制剂中没有观察到明显的效果。硫辛酰胺脱氢酶：心肌黄酶的比率可作为一种变性的量度。
单位定义
一个单位的心肌黄酶将在25℃、pH 7.5下每分钟氧化1.0微摩尔的β-NADH，并相应还原适当的电子受体。
外形
以含有海藻糖冻干粉形式提供。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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