recombinant
expressed in E. coli
Quality Level
form
solid
specific activity
>=30 units/mg protein (biuret)
storage temp.
−20°C
General description
研究领域:癌症
Application
克氏梭菌心肌黄酶已用于:
- 鳃细胞乳酸脱氢酶测定。
- 浸渍在试纸上进行甲酸脱氢酶测定。
- 在电子受体存在下氧化β-NADH或β-NADPH的各种测定。
- 苯醌还原过程中生成半醌。
- 评估培养肌肉细胞中的葡萄糖摄入情况。
- 乳酸脱氢酶细胞毒性检测。
Biochem/physiol Actions
心肌黄酶在电子受体(例如亚甲基蓝或2,6-二氯苯酚吲哚酚)存在下催化β-NADH或β-NADPH的氧化。 心肌黄酶对β-NADH或β-NADPH具有特异性。Straub的猪心脏酶可能具有天然心肌黄酶(β-NADH特异性)以及硫辛酸和硫辛酰胺脱氢酶活性。
Physical form
以含有海藻糖冻干粉形式提供。
Other Notes
一个单位的心肌黄酶将在25℃、pH 7.5下每分钟氧化1.0微摩尔的β-NADH,并相应还原适当的电子受体。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D2197-300UN | 04061832729251 |
| D2197-500UN | 04061833561867 |
| D2197-100UN | 04061832729244 |