D2197
心肌黄酶 来源于克鲁氏梭状芽孢
recombinant, expressed in E. coli, Animal-component free
别名:
心肌黄酶, 硫辛酰胺脱氢酶, 硫辛酰脱氢酶
产品名称
心肌黄酶 来源于克鲁氏梭状芽孢, recombinant, expressed in E. coli, Animal-component free
recombinant
expressed in E. coli
form
solid
specific activity
>=30 units/mg protein (biuret)
storage temp.
−20°C
Quality Level
Application
克氏梭菌心肌黄酶已用于：
- 鳃细胞乳酸脱氢酶测定。
- 浸渍在试纸上进行甲酸脱氢酶测定。
- 在电子受体存在下氧化β-NADH或β-NADPH的各种测定。
- 苯醌还原过程中生成半醌。
- 评估培养肌肉细胞中的葡萄糖摄入情况。
- 乳酸脱氢酶细胞毒性检测。
Biochem/physiol Actions
心肌黄酶在电子受体（例如亚甲基蓝或2，6-二氯苯酚吲哚酚）存在下催化β-NADH或β-NADPH的氧化。 心肌黄酶对β-NADH或β-NADPH具有特异性。Straub的猪心脏酶可能具有天然心肌黄酶（β-NADH特异性）以及硫辛酸和硫辛酰胺脱氢酶活性。
General description
研究领域：癌症
Other Notes
一个单位的心肌黄酶将在25℃、pH 7.5下每分钟氧化1.0微摩尔的β-NADH，并相应还原适当的电子受体。
Physical form
以含有海藻糖冻干粉形式提供。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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