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MAK137

磷脂酶 D 活性检测试剂盒

Name: 磷脂酶 D 活性检测试剂盒
Brand: SIGMA
Price: 5662.03 CNY

sufficient for 100 colorimetric or fluorometric tests

别名:

PLD 活性检测试剂盒

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10 μG

¥5,662.03

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10 μG

¥5,662.03

About This Item

UNSPSC代码:

12161503

NACRES:

NA.84

¥5,662.03

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一般描述

磷脂酶 D (PLD) 催化甘油磷脂的磷酸二酯键水解生成磷脂酸和胆碱。它被广泛表达，参与免疫功能和癌症抑制。[1]PLD 表达异常与人类癌症相关，如乳腺癌、肾癌、胃癌和结直肠癌。[2]

应用

适于检测生物样品中的磷脂酶 D 的活性。本试剂盒可用于研究各种化合物对磷脂酶 D 活性的影响。

采用磷脂酶 D (PLD) 活性检测试剂盒测定了小鼠肝脏和肝脏溶酶体中 PLD 活性。[3]

特点和优势

兼容高通量的操作系统。

适用性

适用于磷脂酶 D 活性的定量测定及药物对磷脂酶 D 代谢影响的评价。

原理

在本试验中，PLD 将磷脂酰胆碱水解为胆碱，使用胆碱氧化酶进行测定，得到比色 (570 nm)/荧光（λex = 530/λem = 585 nm）乘积，与样品中 PLD 活性成正比。单位定义：在试验条件下 (pH 7.4)，1 个单位的 PLD 每分钟催化 1 mmol 胆碱的形成。

象形图

GHS08

警示用语：

Danger

危险声明

H334,H412

预防措施声明

P261 - P273 - P284 - P501

危险分类

Aquatic Chronic 3 - Resp. Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

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Phospholipase D.

Su W and Michael A F

Handbook of Cell Signaling, 1167-1176 (2009)

Phospholipase D inhibition by hexanal is associated with calcium signal transduction events in raspberry.

Walid El Kayal et al.

Horticulture research, 4, 17042-17042 (2017-11-09)

Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient

Altered Metabolism of Phospholipases, Diacylglycerols, Endocannabinoids, and N-Acylethanolamines in Patients with Mastocytosis.

Anne Lise Ferrara et al.

Journal of immunology research, 2019, 5836476-5836476 (2019-07-30)

Mastocytosis is a condition characterized by the expansion and accumulation of mast cells (MCs) in various organs. The symptoms are related to the increased release of MC-derived mediators that exert local and distant effects. MCs are a source and target

Trichoderma harzianum diminished oxidative stress caused by 2,4- dichlorophenoxyacetic acid (2,4-D) in wheat, with insights from lipidomics.

Przemysław Bernat et al.

Journal of plant physiology, 229, 158-163 (2018-08-11)

2,4-dichlorophenoxyacetic acid (2,4-D) is among the most commonly used herbicides applied for weed control during wheat cultivation. However, its application could affect wheat growth. The present study investigates the effect of the ascomycetous fungus Trichoderma harzianum on lipid peroxidation, phospholipids

Structures of three ependymin-related proteins suggest their function as a hydrophobic molecule binder.

Jeong Kuk Park et al.

IUCrJ, 6(Pt 4), 729-739 (2019-07-19)

Ependymin was first discovered as a predominant protein in brain extracellular fluid in fish and was suggested to be involved in functions mostly related to learning and memory. Orthologous proteins to ependymin called ependymin-related proteins (EPDRs) have been found to

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此商品	SRP6166	H1404	SRP6253
SRP6014 HGF human	SRP6166 HGF human	H1404 Hepatocyte Growth Factor human	SRP6253 EGF human
biological source human	biological source human	biological source human	biological source human
recombinant expressed in CHO cells	recombinant expressed in HEK 293 cells	recombinant expressed in Baculovirus infected High-5 cells	recombinant expressed in HEK 293 cells
technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
assay ≥97% (HPLC), ≥97% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay >95% (SDS-PAGE)	assay ≥97% (SDS-PAGE)
mol wt 80.017 kDa	mol wt 70 kDa (single chain, glycosylated)	mol wt ~80 kDa	mol wt calculated mol wt 6.3 kDa, observed mol wt 6.3 kDa by SDS-PAGE (DTT-reduced.)
form lyophilized	form lyophilized	form lyophilized powder	form lyophilized powder

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磷脂酶 D 活性检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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