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用途
sufficient for 100 colorimetric or fluorometric tests
检测方法
colorimetric
fluorometric
相关疾病
cancer
储存温度
−20°C
基因信息
human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)
mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)
rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)
一般描述
磷脂酶 D (PLD) 催化甘油磷脂的磷酸二酯键水解生成磷脂酸和胆碱。它被广泛表达，参与免疫功能和癌症抑制。PLD 表达异常与人类癌症相关，如乳腺癌、肾癌、胃癌和结直肠癌。
应用
适于检测生物样品中的磷脂酶 D 的活性。 本试剂盒可用于研究各种化合物对磷脂酶 D 活性的影响。
采用磷脂酶 D (PLD) 活性检测试剂盒测定了小鼠肝脏和肝脏溶酶体中 PLD 活性。
特点和优势
兼容高通量的操作系统。
适用性
适用于磷脂酶 D 活性的定量测定及药物对磷脂酶 D 代谢影响的评价。
原理
在本试验中，PLD 将磷脂酰胆碱水解为胆碱，使用胆碱氧化酶进行测定，得到比色 (570 nm)/荧光（λex = 530/λem = 585 nm）乘积，与样品中 PLD 活性成正比。单位定义：在试验条件下 (pH 7.4)，1 个单位的 PLD 每分钟催化 1 mmol 胆碱的形成。
警示用语：
Danger
危险声明
危险分类
Aquatic Chronic 3 - Resp. Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
含少量动物源组分生物产品
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Phospholipase D.
Su W and Michael A F
Handbook of Cell Signaling, 1167-1176 (2009)
Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.
Scott S A, et al.
Nature Chemical Biology, 5(2), 108-108 (2009)
Karthika Sriskantharajah et al.
Plants (Basel, Switzerland), 10(11) (2021-11-28)
'Honeycrisp' (Malus domestica Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of
Anne Lise Ferrara et al.
Journal of immunology research, 2019, 5836476-5836476 (2019-07-30)
Mastocytosis is a condition characterized by the expansion and accumulation of mast cells (MCs) in various organs. The symptoms are related to the increased release of MC-derived mediators that exert local and distant effects. MCs are a source and target
Walid El Kayal et al.
Horticulture research, 4, 17042-17042 (2017-11-09)
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient
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