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主要文件

B2025

Sigma-Aldrich

亮蓝 G-胶体浓缩液

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

表单

solution

质量水平

200

储存温度

room temp

相关类别

一般描述

考马斯亮蓝染色剂在十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS PAGE) 中被广泛用于蛋白质的检测。亮蓝 G 染色因其灵敏度接近银染法而被称为蓝银染色。考马斯亮蓝 G 染色液通过疏水作用与蛋白质结合。它可以有效染色角膜前囊膜，毒性相对较小。

应用

亮蓝 G-胶体浓缩液用于在分析酿酒酵母中的
  • 转录因子（TFIID）复合物前，进行体外胃蛋白酶消化后卵巢肿瘤液蛋白质组中
    • 的蛋白质片段的染色

    • 重悬

    用 800 mL 水稀释至 1 L 后，混悬液将含有 0.1% (w/v) 亮蓝 G、0.29 M 磷酸和 16% 饱和硫酸铵。

    分析说明

    已在 SDS-PAGE 上检测了本品的适用性。

    相关产品

    产品编号
    说明
    价格

    象形图

    Corrosion
    GHS05

    警示用语：

    Warning

    危险声明

    H290,H315,H319

    危险分类

    Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

    储存分类代码

    8B - Non-combustible corrosive hazardous materials

    WGK

    WGK 2

    闪点(°F)

    Not applicable

    闪点(°C)

    Not applicable

    从最新的版本中选择一种：

    分析证书(COA)

    Lot/Batch Number
    SLCQ0016
    SLCJ6088
    SLCC7506
    SLBT3592
    SLBQ0154V

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    如果您需要特殊版本，可通过批号或批次号查找具体证书。

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    在文件库中查找您最近购买产品的文档。

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How would you recommend that I reconstitute Product B2025, Brilliant Blue G?

      One bottle of the product makes 1 liter of working solution.  The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.

    4. What is the solution stability of Product B2025, Brilliant Blue G?

      Once diluted the solution should be stable indefinitely.  It should be stored in the refrigerator and shaken well before each use.  The solution tends to precipitate on standing and heat accelerates precipitation.  Therefore, do not leave the bottle at room temperature overnight.

    5. Can you use Product B2025, Brilliant Blue G, with nitrocellulose?

      Use with nitrocellulose is not recommended, due to the methanol.

    6. How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?

      The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.

    7. What is the detection limit of Product B2025, Brilliant Blue G?

      The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    9. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    10. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Roy Auty et al.
    The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
    The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
    Purification of Active TFIID from Saccharomyces cerevisiae EXTENSIVE PROMOTER CONTACTS AND CO-ACTIVATOR FUNCTION
    Auty R, et al.
    The Journal of biological chemistry, 279(48), 49973-49981 (2004)
    A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
    Thomas K, et al.
    Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
    Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
    Dyballa N and Metzger S
    Journal of Visualized Experiments (2009)
    Antonis Ververis et al.
    Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
    Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
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    实验方案

    Brilliant Blue G – Colloidal Concentrate Protocol

    Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

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