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Merck
CN

B0149

Sigma-Aldrich

酸性蓝83

Dye content ~50 %, Powder

别名:

Coomassie 亮蓝R, 亮吲哚蓝蛋白 6B, 酸性蓝 83

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About This Item

经验公式(希尔记法):
C45H44N3NaO7S2
CAS号:
分子量:
825.97
颜色索引号:
42660
Beilstein:
5718025
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

product name

酸性蓝83, Dye content ~50 %, Technical grade

质量水平

形式

powder

组成

Dye content, ~50%

溶解性

H2O: 10 mg/mL

适用性

suitable for detection of protein bands following electrophoresis.

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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一般描述

亮蓝R(C.l.42660)是一种三氨基三芳基甲烷染料,其中两个氨基被苄基化,一个氨基被苯基化。它也被称为亮吲哚菁6B和考马斯蓝R250。

应用

亮蓝R已广泛用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)后的蛋白条带检测。其他应用包括:
  • 用作环氧树脂包埋组织切片蛋白染色剂
  • 定量显微密度测定切片内的蛋白质
  • 染色单体、肌动蛋白微丝,神经突和精子顶体显影。
电泳后检测蛋白条带。

法律信息

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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