Antibody Phage Display

This book covers the protocols highlighting the latest gains in antibody phage display which have made it the definitive technology for monoclonal antibody production. The contributors provide protocols with simplicity and clarity that cover the broad scope of the technology including the key areas of library construction, screening, antibody modification, and expression. The protocols will allow for new opportunities in immunotherapy, drug discovery, and functional genomics.

Overview of Antibody Phage Display Technology and its Applications,
Standard Protocols for the Construction of Fab Libraries,
Standard Protocols for the Construction of scFv Libraries,
Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice,
Construction of Large Naive Fab Libraries,
Construction of Polyclonal Antibody Libraries Using Phage Display,
Antigen-Driven Stimulation of B Lymphocytes In Vitro,
The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion, Panning of Antibody Phage Display Libraries: Standard Protocols, .
Selection of Antibodies Against Biotinylated Antigens,
Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries,
Blocking Immunodominant Epitopes by Competitive Deselection,
Rescue of a Broader Range of Antibodies Using an Epitope-Masking Strategy,
Screening of Phage-Expressed Antibody Libraries by Capture Lift,
Antibody-Guided Selection Using Capture Sandwich ELISA,
Proximity-Guided (ProxiMol) Antibody Selection,
Isolation of Human mAbs Using Guided Selection With Mouse mAbs,
Selecting Antibody to Cell Surface Antigens Using Magnetic Sorting Techniques,
Isolation of Human Tumor-Associated Cell Surface Antigen binding scFv Fragments,
Subtractive Isolation of Single Chain Antibodies Using Tissue Fragments,
Selection of Antibodies Based on Antibody Kinetic Binding Properties,
Selection of Functional Antibodies on the Basis of Valency,
Two-Step Strategy for Alteration of Immunoglobulin Specificity by in vitro Mutagenesis,
Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement, Use of Error-Prone PCR for the Modification of Single-Chain Fv Fragments,
Use of E. coli Mutator Cells to Mature Antibodies,
Chain Shuffling to Modify the Properties of Recombinant Immunoglobulins,
Generation of Bispecific and Tandem Diabodies,
High Level Periplasmic Expression and Purification of scFv Fragments,
Periplasmic Expression and Purification of Recombinant Fab Fragments,
Expression of Antibody Fragments in Pichia pastoris,
Expression of VHH Antibody Fragments in Saccharomyces Cerevisiae,
Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments,
Expression of scFvs and scFv-Fusion Proteins in Eukaryotic Cells,
Expression of Antibody Fabs Fragments and Whole Ig in Mammalian Cells,