跳转至内容
Merck
CN
所有图片(1)

主要文件

安全信息

Z701858

Sigma-Aldrich

Antibody Engineering: Methods and Protocols

登录查看公司和协议定价


About This Item

ISBN-10:
1-58829-092-1
ISBN-13:
978-1-58829-092-2
UNSPSC代码:
55101509

出版信息

B. Lo, ed., Humana Press, 2004, 576 pp., hard cover

一般描述

A collection of techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Included are procedures on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a variety of in vivo applications. Technologies are described for the characterization of antigen-binding affinity and specificity.

目录

PART I. Antibody Sequence and Structure
Internet Resources for the Antibody Engineer
The Kabat Database and a Bioinformatics Example
IMGT, The International ImMunoGeneTics Information System, http://imgt.cines.fr
Antibody Variable Regions: Toward a Unified Modeling Method
Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
PART II. Antibody-Lead Generation
PCR Cloning of Human Immunoglobulin Genes
Antibody Humanization by CDR Grafting
Selection of Human Antibodies from Phage Display Libraries
Production of Human Single-Chain Antibodies by Ribosome Display
Production of Human Antibodies from Transgenic Mice
Selection of Internalizing Antibodies for Drug Delivery
Engineering Multivalent Antibody Fragments for In Vivo Targeting
Production of Recombinant Bispecific Antibodies
PART III. Antibody Expression and Optimization
Expression and Isolation of Recombinant Antibody Fragments in E. coli
Expression of Recombinant Antibodies in Mammalian Cell Lines
Human Antibody Production Using Insect-Cell Expression Systems
Antibody Production in Transgenic Plants
Directed Mutagenesis of Antibody Variable Domains
Antibody Affinity Maturation by Chain Shuffling
Antibody Affinity Maturation by Random Mutagenesis
Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
PART IV. Antibody Characterization and Novel Applications
Antibody Purification by Column Chromatography
Affinity Measurement Using Surface Plasmon Resonance
Kinetic Exclusion Assays to Study High Affinity Binding Interactions in Homogeneous Solutions
Characterization of Antibody–Antigen Interactions by Fluorescence Spectroscopy
Antibody Epitope Mapping Using Arrays of Synthetic Peptides
Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics
Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy
Production and Characterization of Anti-Cocaine Catalytic Antibodies
Recombinant Immunotoxins in the Treatment of Cancer
Antibodies in Proteomics
Targeting of Antibodies Using Aptamers
Index

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助,请联系 客户支持

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门