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产品名称
Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)
重组
expressed in Nicotiana (tobacco)
质量水平
描述
recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)
产品线
BioUltra
方案
≥98% (SDS-PAGE)
表单
powder
比活
≥5 TIU/mg protein
分子量
~_6.5 kDa
技术
inhibition assay: suitable
溶解性
0.85% sodium chloride: 5 mg/mL
UniProt登记号
储存温度
2-8°C
基因信息
cow ... PTI(404172)
相关类别
单位定义
制备说明
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How does Product A6103, Aprotinin bovine, compare to Trasylol?
Trasylol is the Bayer AG registered trademark name for this material.
Do you assay this the activity of Product A6103, Aprotinin bovine, in TIU or in KIU?
We express the product's activity in TIU (Trypsin Inhibitor Units). Multiply the activity on the Certificate of Analysis by 1300 to convert it to KIU (Kallikrein Inhibitor Units).
How should I store solutions of Product A6103, Aprotinin bovine?
We generally advise storing stock solutions of approximately 5-10 mg/mL. Working solutions down to 0.065 micrograms/mL can be stored refrigerated at 2-8°C for one week, or at - 20°C for appoximately six months in single use aliquots. See Table 2 in the Datasheet for details.
At what concentration should I use Product A6103, Aprotinin bovine?
For most applications, 10 to 800 nM works well (based on a molecular weight of 6512 g/mol). This information may be found in the technical bulletin for the Protease Inhibitor Panel, Product No. INHIB1.
Product A6103, recombinant bovine aprotinin has a specification of ~60% protein. What is the remaining components of the solid?
Product A6103, recombinant bovine aprotinin is >98% purity by SDS-PAGE. The product is ~60% protein with the balance being salts (NaCl).
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商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
实验方案
抑肽酶是一种牛胰蛋白酶抑制剂。在测定抑肽酶活性时，该测定使用了在405 nm下的连续分光光度法进行测定。
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
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