product name
抑肽酶 来源于牛肺, BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)
生物来源
bovine lung
质量水平
产品线
BioUltra
检测方案
≥98% (SDS-PAGE)
形式
powder
比活
3-8 TIU/mg solid
分子量
~6,500
技术
inhibition assay: suitable
溶解性
H2O: 5 mg/mL, clear to very slightly hazy
UniProt登记号
储存温度
2-8°C
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
基因信息
cow ... PTI(404172)
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相关类别
应用
抑肽酶被大量用作胰蛋白酶抑制剂。
生化/生理作用
抑肽酶是一种竞争性丝氨酸蛋白酶抑制剂，可与酶的活性位点组成稳定的复合酶并同时对其进行封闭。 这种结合是可逆的，而且绝大多数抑肽酶-蛋白酶复合物可在极端的pH >10 or < 3水平时发生解离。 从结构上来说，抑肽酶是一种来自牛肺的单体球状蛋白，由58个氨基酸组成并排列在具有三个交联二硫键的单个多肽链中。
单位定义
一个胰蛋白酶抑制剂单位 (TIU) 将使 2 个胰蛋白酶单位的活性下降 50%，其中 1 个胰蛋白酶单位将在 25°C pH 7.8 下每分钟水解 1.0 μmol 的 N-α-苯甲酰-DL-精氨酸对硝基苯胺 (BAPNA)。
一个胰蛋白酶抑制剂单位（TIU）可将两个胰蛋白酶单位的活性降低50％，而其中一个胰蛋白酶单位在pH 7.8和25℃下可每分钟水解1.0 μ摩尔的N-α-苯甲酰基-DL-精氨酸对硝基苯胺。另一种常用的单位是KIU，而1 TIU = 1,300 KIU。
制备说明
进一步纯化 A1153，得到活性和纯度略高的产物
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
实验方案
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
抑肽酶是一种牛胰蛋白酶抑制剂。在测定抑肽酶活性时，该测定使用了在405 nm下的连续分光光度法进行测定。
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