推荐产品
生物来源
bovine lung
质量水平
形式
lyophilized powder
比活
3-8 TIU/mg solid
分子量
~6,500
溶解性
H2O: ≥5 mg/mL
UniProt登记号
储存温度
2-8°C
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
基因信息
cow ... PTI(404172)
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一般描述
应用
- 用作放射免疫沉淀测定缓冲液(RIPA)中的蛋白酶抑制剂，用于心脏微血管内皮细胞(CMECs)(4)和乳腺上皮细胞的均质化
- 成纤维细胞血管生成测定
- 唾液上清液的蛋白质组学稳定
生化/生理作用
单位定义
制备说明
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
When using Product A1153, Aprotinin from bovine lung, what is the conversion from KIU to TIU?
According to data from our labs here at Sigma, there are approximately 1300 KIU per 1 TIU. This, along with other useful information, can be found on the product information sheet available at our website: A1153 Product Information Sheet
Is Aprotinin from bovine lung, Product A1153, the same as Trasylol?
Trasylol is the Bayer AG registered trademark name for this material.
How should I store solutions of Aprotinin from bovine lung, Product A1153?
Sterile solutions of aprotinin may be stored at 4 °C or as frozen aliquots at -20 °C.
What are typical concentrations of Aprotinin from bovine lung, Product A1153, to use as a protease inhibitor?
Typical concentration of Aprotinin to be used as a protease inhibitor is 1 μM or approximately 6.5 μg/ml.
What type of inhibitor is Aprotinin from bovine lung, Product A1153?
Aprotinin is a competitive serine protease inhibitor which forms stable complexes that block the active site of the enzyme.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
实验方案
抑肽酶是一种牛胰蛋白酶抑制剂。在测定抑肽酶活性时，该测定使用了在405 nm下的连续分光光度法进行测定。
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
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