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Merck
CN

96992

Sigma-Aldrich

细胞计数试剂盒 - 8

for quantitation of viable cell number in proliferation and cytotoxicity assays

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About This Item

UNSPSC代码:
12352108
NACRES:
NA.32

质量水平

保质期

limited shelf life, expiry date on the label

运输

ambient

储存温度

2-8°C

相关类别

应用

Cell Counting Kit-8 (CCK-8)细胞计数试剂盒采用WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)实现快速检测,其原理是WST-8能在电子载体1-甲氧基PMS的作用下被还原成水溶性甲臜产物。CCK-8溶液可直接加入到细胞中,不需要与其它组分预混。WST-8被细胞内脱氢酶生物还原后生成的橙色甲臜染料能够溶解在组织培养基中,生成的甲臜量与活细胞数量成正比。由于CCK-8溶液非常稳定并且细胞毒性很小,所以可以进行长时间孵育,例如孵育24-48小时。
Cell Counting Kit-8可以对细胞增殖和细胞毒性实验中活细胞数目进行敏感的比色法测定。检测灵敏度高于任何其它四唑盐,如 MTT、XTT或MTS。

相关产品

象形图

Health hazardExclamation mark

警示用语:

Warning

危险分类

Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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分析证书(COA)

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Tetsuro Masuda et al.
Scientific reports, 5, 9170-9170 (2015-03-17)
Bone metastasis of breast cancer cells is a major concern, as it causes increased morbidity and mortality in patients. Bone tissue-derived CXCL12 preferentially recruits breast cancer cells expressing CXCR4 to bone metastatic sites. Thus, understanding how CXCR4 expression is regulated
Takashi Nojiri et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), 4086-4091 (2015-03-17)
Most patients suffering from cancer die of metastatic disease. Surgical removal of solid tumors is performed as an initial attempt to cure patients; however, surgery is often accompanied with trauma, which can promote early recurrence by provoking detachment of tumor
Xiao Deng et al.
Scientific reports, 7, 40256-40256 (2017-01-17)
Endothelial dysfunction and monocyte adhesion to vascular endothelial cells are two critical steps in atherosclerosis development, and emerging evidence suggests that protein sialylation is involved in these processes. However, the mechanism underlying this phenomenon remains incompletely elucidated. In this study
Rohit Chugh et al.
Science translational medicine, 4(156), 156ra139-156ra139 (2012-10-19)
Pancreatic cancer is one of the most lethal human malignancies with an all-stage 5-year survival frequency of <5%, which highlights the urgent need for more effective therapeutic strategies. We have previously shown that triptolide, a diterpenoid, is effective against pancreatic
Leo Scheller et al.
Nature communications, 11(1), 3085-3085 (2020-06-20)
Orthogonal tools for controlling protein function by post-translational modifications open up new possibilities for protein circuit engineering in synthetic biology. Phosphoregulation is a key mechanism of signal processing in all kingdoms of life, but tools to control the involved processes

商品

基于细胞的细胞增殖检测(BrdU、MTT、WST1)、细胞活力和细胞毒性实验,用于癌症、神经科学和干细胞研究应用。

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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