跳转至内容
Merck
CN

96992

Sigma-Aldrich

细胞计数试剂盒 - 8

for quantitation of viable cell number in proliferation and cytotoxicity assays

登录查看公司和协议定价


About This Item

UNSPSC代码:
12352108
NACRES:
NA.32

质量水平

保质期

limited shelf life, expiry date on the label

运输

ambient

储存温度

2-8°C

相关类别

应用

Cell Counting Kit-8 (CCK-8)细胞计数试剂盒采用WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)实现快速检测,其原理是WST-8能在电子载体1-甲氧基PMS的作用下被还原成水溶性甲臜产物。CCK-8溶液可直接加入到细胞中,不需要与其它组分预混。WST-8被细胞内脱氢酶生物还原后生成的橙色甲臜染料能够溶解在组织培养基中,生成的甲臜量与活细胞数量成正比。由于CCK-8溶液非常稳定并且细胞毒性很小,所以可以进行长时间孵育,例如孵育24-48小时。
Cell Counting Kit-8可以对细胞增殖和细胞毒性实验中活细胞数目进行敏感的比色法测定。检测灵敏度高于任何其它四唑盐,如 MTT、XTT或MTS。

相关产品

象形图

Health hazardExclamation mark

警示用语:

Warning

危险分类

Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Tetsuro Masuda et al.
Scientific reports, 5, 9170-9170 (2015-03-17)
Bone metastasis of breast cancer cells is a major concern, as it causes increased morbidity and mortality in patients. Bone tissue-derived CXCL12 preferentially recruits breast cancer cells expressing CXCR4 to bone metastatic sites. Thus, understanding how CXCR4 expression is regulated
Xiao Deng et al.
Scientific reports, 7, 40256-40256 (2017-01-17)
Endothelial dysfunction and monocyte adhesion to vascular endothelial cells are two critical steps in atherosclerosis development, and emerging evidence suggests that protein sialylation is involved in these processes. However, the mechanism underlying this phenomenon remains incompletely elucidated. In this study
Takashi Nojiri et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), 4086-4091 (2015-03-17)
Most patients suffering from cancer die of metastatic disease. Surgical removal of solid tumors is performed as an initial attempt to cure patients; however, surgery is often accompanied with trauma, which can promote early recurrence by provoking detachment of tumor
Xianglong Yi et al.
Molecular vision, 17, 673-679 (2011-03-16)
1,25-Dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] has recently been found to be involved in the development of autoimmune diseases. This study was to investigate the expression and potential role of 1,25(OH)(2)D(3) in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Blood samples were obtained from
Rohit Chugh et al.
Science translational medicine, 4(156), 156ra139-156ra139 (2012-10-19)
Pancreatic cancer is one of the most lethal human malignancies with an all-stage 5-year survival frequency of <5%, which highlights the urgent need for more effective therapeutic strategies. We have previously shown that triptolide, a diterpenoid, is effective against pancreatic

商品

基于细胞的细胞增殖检测(BrdU、MTT、WST1)、细胞活力和细胞毒性实验,用于癌症、神经科学和干细胞研究应用。

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门