应用
该活/死细胞复染试剂盒可同时对活细胞和死细胞进行荧光染色。该试剂盒包含钙黄绿素-AM和碘化丙啶（PI）两种溶液，分别用于染色活细胞和死细胞。其中钙黄绿素-AM，即钙黄绿素的乙酰羟甲基酯，具有高度亲脂性和细胞膜透过性。尽管钙黄绿素-AM自身并非荧光分子，但活细胞中的酯酶可以催化钙黄绿素-AM生成钙黄绿素，从而发出强烈的绿色荧光(λex 490 nm, λem 515 nm). 因此钙黄绿素-AM只能染色活细胞。而核染色染料PI不能透过活细胞的细胞膜。但可以透过死细胞膜的变性区域来到达细胞核，并与细胞中的DNA双螺旋结构结合，从而发出红色荧光(λex 535 nm, λem 617 nm)。钙黄绿素和PI-DNA都能被490nm波长的光激发，因此可以采用荧光显微镜同时监测活细胞和死细胞。使用λex激发光时只能观察到死亡细胞。
仅试剂盒组分
产品编号
说明
- Solution A (Calcein AM solution) 4 × 50
- Solution B (propidium iodide solution) 300 μL
相关产品
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说明
价格
WGK
WGK 2
闪点(°F)
185.0 °F
闪点(°C)
85 °C
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基于细胞的细胞增殖检测（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，用于癌症、神经科学和干细胞研究应用。
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