技术
HPLC: suitable
试验参数
column: Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)
mobile phase: 2 mM ammonium formate in 90:10 (v:v) acetonitrile:water
flow rate: 0.3 mL/min
column temp.: 55 °C
detector: TOF/MS
injection: 2 μL
sample: 400 ng/mL in acetonitrile
适用性
application for HPLC
应用
clinical testing
forensics and toxicology
分析说明
The rapid analysis by HPLC with TOF-MS detection of seven opioids is shown here using an Ascentis Express HILIC column. Cerilliant CRMs were used to ensure reliable MS identification and quantification.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Oxycodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
6-Acetylcodeine solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
6-Acetylmorphine, 1.0 mg/mL solution, 1 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Codeine solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Hydrocodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Morphine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Hydromorphone solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
分析色谱柱
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