LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis^® Express C18

技术

HPLC: suitable

试验参数

sample/matrix: Plasma proteins were removed with addition of 900 μL of acetonitrile, containing deuterated internal standards, to 250 μL of human EDTA plasma. The mixture was vortexed, (centrifuged and the supermatant evaporated before being reconstituted in a 50:50 solution of methanol and water. 10 μL (corresponding to 8.57 μL of plasma) was injected into the HPLC.)
column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A]: Water (5 mM ammonium acetate and 0.012% formic acid); [B]: Methanol (5 mM ammonium acetate and 0.012% formic acid)
gradient: 70 to 95% B in 10 minutes held 4 minutes
flow rate: 0.6 mL/min
column temp.: 40 °C
detector: ESI(-), MRM Mode

适用性

application for HPLC

应用

clinical