Volatile Fraction of Roasted Coffee Beans Using SPME on Omegawax^™ 250

技术

gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable

试验参数

sample/matrix: 2 g of coffee beans
SPME fiber: 50/30 μm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U)
extraction: headspace, with a pre-equilibration period of 10 min. at 60 °C, and then a fiber exposure of 40 min. at 60 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm
desorption process: 260 °C for 5 min
column: Omegawax 250, 30 m x 0.25 mm I.D., 0.25 μm (24136)
oven: 40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280 °C (2 min)
inj. temp.: 260 °C
detector: MS, m/z 40-400
carrier gas: helium, 34.8 cm/sec constant
injection: splitless
scan range: m/z 40-400
sample: Roasted coffee
liner: 0.75 mm I.D. SPME/Splitless (2633901)

适用性

application for SPME

应用

food and beverages