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一般描述
淀粉样蛋白-β(Aβ)肽是阿尔茨海默病大脑的老年斑特征的主要成分。它是一种I型跨膜蛋白。编码淀粉样蛋白-β的基因定位于人21号染色体上。
应用
本品人淀粉样前体蛋白β已用作肽标准品。
生化/生理作用
分泌型β淀粉样蛋白前体(sAPPβ)是β淀粉样蛋白前体(APP)水解得到的裂解产物。APP被β-分泌酶切成2个片段，sAPPβ和β-淀粉样肽(Aβ)。Aβ是阿尔茨海默病(AD)的主要标志之一——淀粉样老年斑的成分，sAPPβ被认为可调节神经元功能和细胞存活。瑞典家系AD中存在的APP突变使APP更易被β-分泌酶切割，形成更多的Aβ和sAPPβ。相反，蛋白激酶C(PKC)激活可降低sAPPβ浓度。反面高尔基体网状结构(TGN)是β-分泌酶活性发挥的主要位点。
外形
0.2 μm过滤溶液，含20 mM Tris(pH 7.4)、0.5 M NaCl、1 mM EDTA、5 mM βME、2 μg/ml抑肽酶
制备说明
sAPPβ在大肠杆菌中表达为可溶性蛋白，并在非变性条件下纯化。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
In vivo BACE1 inhibition leads to brain A? lowering and increased a-secretase processing of APP without effect on Neuregulin-1
Sethu Sankaranarayanan
Journal of Pharmacology and Experimental Therapeutics (2018)
Regulation of global gene expression and cell proliferation by APP.
Wu Y
Scientific Reports (2016)
Conformational Dynamics of Specific A? Oligomers Govern Their Ability To Replicate and Induce Neuronal Apoptosis.
Dean DN
Biochemistry (2016)
C Haass et al.
Nature, 357(6378), 500-503 (1992-06-11)
Progressive cerebral deposition of the amyloid beta-peptide is an early and invariant feature of Alzheimer's disease. The beta-peptide is released by proteolytic cleavages from the beta-amyloid precursor protein (beta APP), a membrane-spanning glycoprotein expressed in most mammalian cells. Normal secretion
D M Skovronsky et al.
The Journal of biological chemistry, 275(4), 2568-2575 (2000-01-25)
The release of amyloidogenic amyloid-beta peptide (Abeta) from amyloid-beta precursor protein (APP) requires cleavage by beta- and gamma-secretases. In contrast, alpha-secretase cleaves APP within the Abeta sequence and precludes amyloidogenesis. Regulated and unregulated alpha-secretase activities have been reported, and the
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