S4195
β-分泌酶 人
recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), extracellular domain, ≥10,000 units/mg protein, ≥90% (SDS-PAGE), buffered aqueous solution
别名:
BACE1, Memapsin-2, 天冬氨酸-2
产品名称
β-分泌酶 人, recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), ≥10,000 units/mg protein
recombinant
expressed in HEK 293 cells (C-terminal FLAG tagged)
description
extracellular domain
assay
≥90% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥10,000 units/mg protein
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
human ... BACE1(23621)
Application
人 β-分泌酶或BACE1已被用于研究淀粉样蛋白前体蛋白与β-分泌酶之间的结合反应。 它也已被用于BACE1分析。
Biochem/physiol Actions
BACE1（β位APP裂解酶1）是一种跨膜天冬氨酰蛋白酶，负责裂解淀粉样前体蛋白 (APP)。BACE1介导的淀粉样前体蛋白 (APP)的裂解是致病性淀粉样β肽生成过程中的第一步，因此使其成为阿尔茨海默氏病 (AD)的主要药物靶标。在患有轻度认知障碍（MCI）和AD的患者的脑脊液中发现BACE1的酶活性水平升高，从而表明BACE1酶活性在MCI向AD转化中的重要性。
跨膜蛋白酶负责淀粉样蛋白前体蛋白(APP)的β位点裂解，从而产生淀粉样蛋白β肽。
BACE1（β-分泌酶或β-位点APP裂解酶）是一种跨膜蛋白酶，负责淀粉样前体蛋白（APP）的β位点裂解，从而产生淀粉样 β 淀粉样蛋白肽（Aβ）。大脑中Aβ的积累是阿尔茨海默氏症进展的主要原因，因此BACE1是发现抑制剂药物的靶标。
Physical form
溶于 20 mM Hepes，125 mM NaCl (pH 7.4中。
Other Notes
在37℃温度，pH4.5下，一个单位1分钟将水解1.0甲酚7-甲氧基香豆素-4-乙酰基-[Asn670, Leu671]--淀粉样β/ A4前体蛋白770片段667-676-（2,4-二硝基苯基）-Lys-Arg -Arg酰胺底物。
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
cceleration of deposition of peptide on ultrasonically formed nucleus studied by wireless quartz-crystal-microbalance biosenso
Hirotsugu Ogi
Biosensors, 40(1) (2013)
Acceleration of deposition of peptide on ultrasonically formed nucleus studied by wireless quartz-crystal-microbalance biosensor
Hirotsugu Ogi
Biosensors And Bioelectronics (2013)
Roland Nicsanu et al.
Journal of Alzheimer's disease : JAD, 87(1), 433-441 (2022-03-12)
Beta-site APP cleaving enzyme 1 (BACE1) is the rate-limiting enzyme in amyloid-β (Aβ) plaques formation. BACE1 activity is increased in brains of patients with Alzheimer's disease (AD) and mild cognitive impairment (MCI) and plasma levels of BACE1 appears to reflect
Platelet-Derived Secreted Amyloid-Precursor Protein-? as a Marker for Diagnosing Alzheimer?s Disease
Current neurovascular research (2013)
Carlo Cervellati et al.
GeroScience, 42(1), 159-167 (2019-11-21)
Beta-secretase (BACE1) is a key enzyme in the formation of amyloid-β; its activity/concentration is increased in brain and cerebrospinal fluid of patients with late-onset Alzheimer's disease (LOAD). Since BACE1 was found also in blood, we evaluated its potential as peripheral
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