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产品名称
Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 95-100 kDa
种属反应性
human, rat, mouse
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain
microarray: suitable
western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... APP(351)
mouse ... App(11820)
rat ... App(54226)
一般描述
Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. It is expressed at high levels in the brain. APP gene is mapped to human chromosome 21q11.2-q21. It is a 695 amino acid protein which possesses a large ectodomain and comparatively short intracellular region.
The immunogen sequence is identical to the APP isoforms APP751 and APP770 and is highly conserved (single amino acid substitution) in rat and mouse APP695. The antibody recognizes APP695, APP751 and APP770.
免疫原
synthetic peptide corresponding to the N-terminal of human APP695 (amino acids 46-60) conjugated to KLH.
应用
Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit has been used in:
- western blotting
- immunostaining
- immunofluorescence
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.
生化/生理作用
Amyloid precursor proteins (APPs) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP serves as a transcriptional regulator and as a receptor for kinesin-1-mediated axonal transport. Alzheimer′s disease is characterized by deposition of amyloid in the central nervous system, in neurite plaques and on cerebral vasculature. Mutations in the APP gene are linked with rare forms of autosomal dominant familial Alzheimer′s Disease (FAD). APPs undergo post-translational processing including N- and O-glycosylation, phosphorylation and sulfation.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Association of TrkA and APP Is Promoted by NGF and Reduced by Cell Death-Promoting Agents
Canu N, et al.
Frontiers in Molecular Neuroscience, 10 (2017)
Dopamine induces apoptosis in APPswe-expressing Neuro2A cells following Pepstatin-sensitive proteolysis of APP in acid compartments
Cagnin M, et al.
Brain Research, 1471, 102-117 (2012)
Homo-and Heterodimerization of Proteins in Cell Signaling: Inhibition and Drug Design
Advances in Protein Chemistry and Structural Biology, 111, 1-59 (2018)
The Alzheimer amyloid precursor protein maps to human chromosome 21 bands q21. 105-q21. 05
Korenberg JR, et al.
Genomics, 5(1), 124-127 (1989)
Migration of blood cells to beta-amyloid plaques in Alzheimer's disease
Hohsfield LA, et al.
Experimental Gerontology, 65, 8-15 (2015)
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