产品名称
红细胞裂解缓冲液Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
Application
建议在细胞颗粒中加入1 mL缓冲液(细胞颗粒=1个脾脏或1-2亿个细胞)。 轻轻混合1分钟。 用15-20mL培养基活盐溶液稀释该缓冲液。 在250-500 × g 离心7分钟并弃去上清。 细胞可被稀释并准备计数或融合。 如果裂解不完全，可重复1-4步。
Biochem/physiol Actions
红细胞裂解缓冲液已被开发用于杂交瘤实验中，用于在融合前从小鼠脾细胞悬液中去除红细胞。 在需要从细胞悬液(如全血)中去除红细胞的体系中，它也很有用。
Preparation Note
含8.3 g/L氯化铵的0.01 M Tris-HCl缓冲液。
Other Notes
注：本品用于去除小鼠红细胞。 本品可能不适用于其他动物红细胞的裂解。 该产品在小鼠脾细胞以外的任何应用场合的适用性必须由研究人员确定。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
H Guan et al.
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Osteoclasts are responsible for physiological bone remodeling as well as pathological bone destruction in osteoporosis, periodontitis and rheumatoid arthritis, and thus represent a pharmacological target for drug development. We aimed to characterize and compare the cytokine-induced osteoclastogenesis of bone marrow
Christopher J M Piper et al.
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Regulatory B cells (Bregs) play a critical role in the control of autoimmunity and inflammation. IL-10 production is the hallmark for the identification of Bregs. However, the molecular determinants that regulate the transcription of IL-10 and control the Breg developmental
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R7757-100ML | 04061836696771 |