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R7757

Sigma-Aldrich

红细胞裂解缓冲液Hybri-Max™

Liquid, sterile-filtered, suitable for hybridoma

别名:

RBC裂解缓冲液

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

product name

红细胞裂解缓冲液Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma

无菌性

sterile-filtered

质量水平

400

形式

liquid

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

应用

建议在细胞颗粒中加入1 mL缓冲液(细胞颗粒=1个脾脏或1-2亿个细胞)。 轻轻混合1分钟。 用15-20mL培养基活盐溶液稀释该缓冲液。 在250-500 × g 离心7分钟并弃去上清。 细胞可被稀释并准备计数或融合。 如果裂解不完全，可重复1-4步。

生化/生理作用

红细胞裂解缓冲液已被开发用于杂交瘤实验中，用于在融合前从小鼠脾细胞悬液中去除红细胞。 在需要从细胞悬液(如全血)中去除红细胞的体系中，它也很有用。

组分

含8.3 g/L 氯化铵的0.01 M Tris-HCl 缓冲液。

其他说明

注：本品用于去除小鼠红细胞。 本品可能不适用于其他动物红细胞的裂解。 该产品在小鼠脾细胞以外的任何应用场合的适用性必须由研究人员确定。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Is the BSA standard supplied with Product B6916, Bradford Reagent?

    No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, be used to lyse cells that are trapped in whole tissue preparations?

    Unfortunately, we have not verified the suitability of this lysis buffer for using with tissue samples and do not have a protocol.   

  7. In step 3 of your usage sheet for Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, you say to dilute this buffer with medium or "salt solution". What salt solution is appropriate?

    Any balanced salt solution such as DPBS (Dulbecco's phosphate buffered saline) or HBSS (Hank's Balanced Salt Solution) may be used to dilute the buffer when following this procedure.

  8. Since you do not recommend Product R7757, Red Blood Cell Lysing Buffer Hybri-Max™, for treating whole blood, what protocol and products do you recommend for red cell lysis in a sample of whole blood?

    The following is a suggested protocol:1. Centrifuge to remove plasma. A volume of 0.2 mL of whole blood will yield approximately 0.1 mL of packed cells. Add 0.1ml of 1X buffer such as HBSS (Hanks' balanced salt solution, Product No. H1641) or DPBS (Dulbecco's phosphate buffered saline solution, Product No. D1283), to 0.1 mL of the pelleted cells and resuspend the pellet.2. Add 1-2 mls of 0.1X diluent (or water) and quickly mix cells using the same pipette.3. After 15 seconds, quickly add a volume of 2X diluent in the amount used in step 2 and mix thoroughly.  Dilute further with 1X diluent.  Wash cells once with buffer.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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