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Merck
CN

H0262

Sigma-Aldrich

Hybri-Max™ HAT培养基补充物 (50×)

lyophilized powder, suitable for hybridoma, BioXtra

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别名:
HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

无菌性

γ-irradiated

产品线

BioXtra

形式

lyophilized powder

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

运输

dry ice

储存温度

−20°C

应用

HAT培养基添加剂(50×) Hybri-Max 已用于:
  • 次黄嘌呤-鸟嘌呤磷酸核糖基转移酶 (HPRT) 缺陷型克隆体的选择和验证
  • mAbs(单克隆抗体)制备
  • 基于荧光素酶的生物测定 (LUC)

重悬

用 10 ml 无菌细胞培养基重悬小瓶的内容物。 储备溶液足以制备 500 ml 培养基。 最终工作浓度:100 μM 氨基喋呤, 0.4 μM 氨基喋呤,16 μM 胸苷

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazardExclamation mark

警示用语:

Danger

危险声明

危险分类

Acute Tox. 4 Oral - Repr. 1B

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


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Geoffrey A Mueller et al.
Journal of immunology (Baltimore, Md. : 1950), 205(8), 1999-2007 (2020-09-11)
IgE Abs drive the symptoms of allergic disease upon cross-linking allergens on mast cells or basophils. If the IgE binding sites on the allergens could be identified, it may be useful for creating new forms of immunotherapy. However, direct knowledge
J H Connolly et al.
Journal of anatomy, 195 ( Pt 2), 161-171 (1999-10-21)
The gross and histological appearance and the distribution of T and B lymphocytes and plasma cells are described for lymphoid tissues obtained from 15 platypuses. The spleen was bilobed and surrounded by a thick capsule of collagen, elastic fibres and
Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies against interferon beta
Hermanrud C, et al.
Journal of Immunological Methods, 1-9 (2016)
Renjian Hu et al.
Viruses, 12(3) (2020-03-25)
At present, the clinical detection method of human papillomavirus (HPV) is mainly based on the PCR method. However, this method can only be used to detect HPV DNA and HPV types, and cannot be used to accurately predict cervical cancer.
The expression and function of the NKRP1 receptor family in C57BL/6 mice
Aust J G, et al.
Journal of Immunology, jimmunol-j0804281 (2009)

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Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.

技术方面的重大进展让单克隆抗体生产更快速高效。现有三个成熟的抗体发现平台。我们提供基于不同技术生产单克隆抗体所需的试剂。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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