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H0137

Sigma-Aldrich

Hybri-Max™ HT培养基补充物 (50×)™

lyophilized powder, suitable for hybridoma, BioXtra

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别名:
HT Supplement, Hypoxanthine-Thymidin (HT) media supplement
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

形式

lyophilized powder

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

储存温度

−20°C

相关类别

应用

Ht培养基补充剂是优质的次黄嘌呤和胸腺嘧啶混合物，可用于杂交瘤筛选和细胞培养应用。HT培养基补充剂 (50×) Hybri-Max™已用作INS-1 β-细胞共培养基的补充剂。 这一成分适合用作杂交瘤细胞培养基以及HeLa S3和MTH1-KO细胞的杜氏改良Eagle培养基（DMEM）。

重悬

10mL 无菌细胞培养基可复原一小瓶本品。储存液足以制备 500mL 培养基。最终工作浓度：100μM 次黄嘌呤、16μM 胸腺嘧啶核苷。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

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检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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