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H0137

Hybri-Max™ HT培养基补充物 (50×)^™

Name: Hybri-Max™ HT培养基补充物 (50×)<SUP>™</SUP>
Brand: SIGMA

lyophilized powder, suitable for hybridoma, BioXtra

别名:

HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

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About This Item

UNSPSC代码:

12352207

NACRES:

NA.75

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Sigma-Aldrich

H0262

Hybri-Max™ HAT培养基补充物 (50×)^™

Roche

11363735001

杂交瘤融合和生长补充剂

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F5881

弗氏完全佐剂

Roche

10783641001

聚乙二醇1500

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F5506

弗氏不完全佐剂

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F2442

胎牛血清

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11088947001

BM Condimed H1

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P7181

聚乙二醇溶液

Sigma-Aldrich

A5159

氨基蝶呤

Sigma-Aldrich

P7306

聚乙二醇/二甲基亚砜溶液

产品名称

Hybri-Max™ HT培养基补充物 (50×)^™, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

表单

lyophilized powder

技术

cell culture | hybridoma: suitable

杂质

endotoxin, tested

储存温度

−20°C

应用

Ht培养基补充剂是优质的次黄嘌呤和胸腺嘧啶混合物，可用于杂交瘤筛选和细胞培养应用。HT培养基补充剂 (50×) Hybri-Max^™已用作INS-1 β-细胞共培养基的补充剂。这一成分适合用作杂交瘤细胞培养基以及HeLa S3和MTH1-KO细胞的杜氏改良Eagle培养基（DMEM）。

重悬

10mL 无菌细胞培养基可复原一小瓶本品。储存液足以制备 500mL 培养基。最终工作浓度：100μM 次黄嘌呤、16μM 胸腺嘧啶核苷。

法律信息

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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分析证书(COA)

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The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress

Hashiguchi K, et al.

Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 808, 10-19 (2018)

Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii

Gondim L, et al.

Experimental Parasitology, 163, 46-56 (2016)

Simulated Cholinergic Reinnervation of beta (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing beta Cell and Cholinergic Cell

Jiao A, et al.

International Journal of Endocrinology, 2018 (2018)

Identification of Dengue Virus Serotype 3 Specific Antigenic Sites Targeted by Neutralizing Human Antibodies.

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Cell host & microbe, 27(5), 710-724 (2020-05-15)

The rational design of dengue virus (DENV) vaccines requires a detailed understanding of the molecular basis for antibody-mediated immunity. The durably protective antibody response to DENV after primary infection is serotype specific. However, there is an incomplete understanding of the

In vitro cultivation of Babesia duncani (Apicomplexa: Babesiidae), a zoonotic hemoprotozoan, using infected blood from Syrian hamsters (Mesocricetus auratus).

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Parasitology research, 118(8), 2409-2417 (2019-06-15)

Human babesiosis, a tick-borne disease similar to malaria, is most often caused by the hemoprotozoans Babesia divergens in Europe, and Babesia microti and Babesia duncani in North America. Babesia microti is the best documented and causes more cases of human

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实验方案

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

主要文件

Hybri-Max™ HT培养基补充物 (50×)^™