一般描述
应用
- 观察内皮素-1（ET-1）和血管紧张素II（Ang-II）处理后原代乳鼠心肌细胞（PNCM）和H9C2细胞（大鼠心肌成肌细胞）F-肌动蛋白的重组情况。
- 在免疫化学中标记微丝。
- 在免疫荧光分析中对F-肌动蛋白进行染色。
生化/生理作用
警示用语：
Danger
危险分类
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I dissolve Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
This product is soluble in methanol, ethanol, butanol and pyridine. It is soluble in cold water at a concentration of 5 mg/mL; it is much more soluble in hot water. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., entry# 7336 (1996). Solutions should be prepared fresh, and protected from light whenever possible.
How do I prepare stock solutions of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1 to 5 mg/mL. Make final dilutions in aqueous physiological buffers for a staining range from 0.1 μM to 100 μM with corresponding incubation times of 15 minutes to 72 hours.
What are the excitation and emission wavelengths used for fluorescent detection of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
Excitation wavelengths of 540-545 nm and emission wavelengths of 570-573 nm can be used. See: Faulstich, H., J. Muscle Res. Cell Motility, 9, 370 (1988), and Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989).
How do I stain cells with Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
A typical application for staining cells can be found in the product information sheet (under Documents, above).
How do I store Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
The product should be stored at a powder in the freezer at -20°C.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
在实验室计时器蜂鸣不止、报告和授权截止期限迫在眉睫时，很容易放松对实验室试剂质量的授权。
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