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MBD0015

Sigma-Aldrich

DAPI成品溶液

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

别名:

4′,6-二脒基-2-苯基吲哚 二盐酸盐, DAPI染料, DAPI染色, DNA含量复染剂, 2-（4-氨基苯基）-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐

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5 G
CN¥482.77
25 G
CN¥531.99
100 G
CN¥995.77

CN¥482.77

预计发货时间April 07, 2025详情

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5 G
CN¥482.77
25 G
CN¥531.99
100 G
CN¥995.77

About This Item

经验公式（希尔记法）:
C16H15N5 · 2HCl
CAS号:
28718-90-3
分子量:
350.25
Beilstein:
4894417
MDL编号:
MFCD00012681
UNSPSC代码:
41105500
NACRES:
NA.55

CN¥482.77

预计发货时间April 07, 2025详情

获取大包装报价

质量水平

200

表单

liquid

储存条件

protect from light

浓度

1 mg/mL

技术

transfection: suitable

溶解性

water: soluble

荧光

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

适用性

suitable for nucleic acid staining

运输

dry ice

储存温度

−20°C

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Amicon® Ultra Centrifugal Filter, 3 kDa MWCO sample volume 2 mL, regenerated cellulose membrane, MWCO 3 kDa

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material

polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

product line

Amicon®

product line

Amicon®

product line

Amicon®

product line

Amicon®

parameter

2 mL sample volume

parameter

2 mL sample volume

parameter

2 mL sample volume

parameter

2 mL sample volume

一般描述

DAPI，4′,6-二氨基-2-苯基吲哚，是一种蓝色荧光核酸染色剂，在选择性结合双链DNA的小沟时会发出明亮的荧光。它对DNA的选择性和高细胞通透性使得对细胞核的染色几乎没有胞质背景干扰。DAPI-DNA复合物的激发和发射波长分别为360 nm和460 nm。

应用

在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。
在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱，通过对双链复合体的特殊可视化检测印迹应用中的退火探针，并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。
提供预制DAPI溶液，浓度为1mg/mL建议用所需的缓冲液以1：1000的比例稀释预制DAPI溶液，制备1 μg/mL的工作浓度（大多数应用中使用的典型工作浓度为0.1 μg/mL-10 μg/mL）。将DAPI溶液（1 mg/mL）避光储存于-20°C。建议分装预制DAPI溶液，并避免反复冻融。

在琼脂糖凝胶中染色DNA，DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱，通过对双链复合体的特殊可视化检测印迹应用中的退火探针，并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

用于免疫荧光显微镜、高含量筛选（HCS）、染色体染色和流式细胞术（FACS）中的核复染色。

生化/生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟（富含AT的DNA优先）结合，形成稳定的复合物，其发出的荧光比单独的DAPI高约20倍。

特点和优势

  • 即用型液体，细胞可渗透染料。
  • 可与活细胞和固定细胞一起使用。
  • 对固定细胞染色和DNA含量定量有效。
  • 在荧光显微镜或高含量筛选（HCS）应用中使用荧光抗体时，可获得最佳复染效果。

外形

提供预制DAPI溶液，水中浓度为1mg/mL

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H317

危险分类

Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000380386
    0000190381
    0000216561
    0000367284
    0000187804

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