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Merck
CN

L9031

脂肪酶 来源于米黑毛霉菌

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

别名:

三酰基甘油脂肪酶, 三酰基甘油酰基水解酶

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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
MDL number:
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产品名称

脂肪酶 来源于米黑毛霉菌, lyophilized powder, ≥4,000 units/mg solid (using olive oil)

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

form

lyophilized powder

specific activity

≥4,000 units/mg solid (using olive oil)

storage temp.

2-8°C

Quality Level

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Biochem/physiol Actions

可水解甘油三酯、甘油二酯、甘油单酯(速率依次递减)。
Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Microbial lipase has a wide industrial application due to its stability, broad substrate specificity, and selectivity. It may be used in the synthesis of biosurfactants and finds application in organic chemical processing, dairy industry, oleochemical industry, detergent formulations, paper, cosmetics, nutrition, and the pharmaceutical industry.

Analysis Note

Protein determined by biuret.

Application

Lipase from Mucor miehei can be used to drive the synthesis of flavor esters.
Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

General description

Lipase is produced by several animals, microbes, and plants. Lipase from Mucor sp. is a fungal lipase. It is a member of the serine hydrolase family.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A review on microbial lipases production
Treichel H, et al.
Food and Bioprocess Technology, 3(2), 182-196 (2010)
Torben Snabe et al.
Chemistry and physics of lipids, 133(1), 37-49 (2004-12-14)
Removal of lipidic molecules from surfaces can be accomplished using detergents containing lipases. Surface cleaning is usually performed under alkaline conditions due to increased solubility of the hydrolysis products, especially free fatty acids. This paper shows that removal of a
H S Ryu et al.
Applied microbiology and biotechnology, 70(3), 321-326 (2005-08-10)
A Photobacterium strain, M37, showing lipolytic activity, was previously isolated from an intertidal flat of the Yellow Sea in Korea and identified as Photobacterium lipolyticum sp. nov. In the present study, the corresponding gene was cloned using the shotgun method.
Design and synthesis of an affinity probe that targets caspases in proteomic experiments
Liau, M-L, et al
Tetrahedron Letters, 44, 1043-1046 (2003)
Hui-da Wan et al.
Applied biochemistry and biotechnology, 166(6), 1454-1462 (2012-01-21)
Microwave has nonthermal effects on enzymatic reactions, mainly caused by the polarities of the solvents and substrates. In this experiment, a model reaction with caprylic acid and butanol that was catalyzed by lipase from Mucor miehei in alkanes or arenes

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