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一般描述
来自猪胰腺的脂肪酶(PPL)或猪胰腺脂肪酶是小型球状蛋白,N-端含有αβ型折叠区域。另含有催化三联体Ser、Asp和His残基和C端β-三明治结构域。猪胰腺脂肪酶属于羧酸酯酶家族的亚家族。
应用
猪胰腺脂肪酶已用于:
- 植物提取物的脂肪酶抑制分析
- 糊化淀粉/纤维混合物的体外消化
- 加入模拟肠液以模拟消化道条件,用于发酵燕麦中的发酵乳杆菌(L. fermentum)PC1的继续存活
脂肪酶在工业上用于拆分手性化合物和生物柴油的酯交换生产。
生化/生理作用
猪胰腺脂肪酶(PPL)可催化脂肪(脂质)水解。它在大多数生物体的膳食脂质(如甘油三酯、脂肪、油脂)消化、转运和加工中起重要作用。猪胰腺脂肪酶具有高性价比,可用于生物转化反应。它具有高选择性,并具有催化活性。猪胰腺脂肪酶还可掺入去污剂,用于去除衣物上的油渍。
脂肪酶可催化水溶液中酯的水解和非水溶液中酯的合成。它们通过水解聚对苯二甲酸乙二酯中的酯键,产生羟基和羧基。
脂肪酶可催化三酰甘油的水解,生成甘油和游离脂肪酸。
脂肪酶可催化三酰甘油的水解,生成甘油和游离脂肪酸。
单位定义
一个酶活性单位是指在pH 7.7、温度37 °C条件下,以橄榄油为底物,在1小时内水解甘油三酸酯而释放1.0微当量的脂肪酸所需的酶量。
分析说明
通过缩二脲法测定的蛋白质。
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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