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H7787

Millipore

HIS-Select®离心柱

别名:

His-Tag Spin Columns

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

一般描述

试剂盒含有针对每种离心柱的3种组合管。

应用

预填充的即用型HIS-Select离心柱可实现对含有组氨酸标签蛋白的小规格细胞粗提取物进行快速而一致的纯化。该离心柱包含二氧化硅颗粒基质并使用HIS-Select专利的不带电荷的亲水性镍螯合键合化学。HIS-Select离心柱对组氨酸标记的蛋白具有高度选择性，而对其他蛋白的结合特异性极低。这些便捷的离心柱可实现快速的一步法纯化，在不到15分钟的时间内分离出至少150 μg的组氨酸标签蛋白。

特点和优势

  • 高选择性确保了高纯度
  • 便捷且即用
  • 每次使用的一致性蛋白结合
  • 兼容CelLytic™细胞裂解试剂

法律信息

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCQ6970
SLCQ5845
SLCL7069
SLCL7076
SLCC2035

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the binding capacity of the HIS-Select® Spin Column, Product H7787?

    The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.

  4. Can HIS-Select® Spin Column, Product H7787, be re-used?

    We do not recommend re-using the columns. Each column is intended for one time use.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Maria A Schumacher et al.
Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Maureen J Bibb et al.
Molecular microbiology, 84(6), 1033-1049 (2012-05-16)
The chaplin and rodlin proteins together constitute the major components of the hydrophobic sheath that coats the aerial hyphae and spores in Streptomyces, and mutants lacking the chaplins are unable to erect aerial hyphae and differentiate on minimal media. We
Wenbo Li et al.
Drug design, development and therapy, 10, 3415-3423 (2016-11-02)
Choroidal neovascularization (CNV) in age-related macular degeneration usually causes blindness. We established a novel targeted inhibitor for CNV in age-related macular degeneration. The inhibitor CR2-sFlt 1 comprises a CR2-targeting fragment and an anti-vascular endothelial growth factor (VEGF) domain (sFlt 1).
Tian Luo et al.
Clinical and vaccine immunology : CVI, 17(1), 87-97 (2009-12-04)
Recently, major species-specific antibody epitopes in three immunoreactive tandem repeat proteins (TRPs) of Ehrlichia chaffeensis, TRP32, TRP47, and TRP120, have been identified and molecularly characterized within tandem repeat (TR) regions. In this study, we mapped the major immunodeterminants of the
Dirk Maass et al.
PloS one, 4(7), e6373-e6373 (2009-07-29)
As the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches that have successfully increased the carotenoid content in agronomically relevant non-green plant tissues through tissue-specific PSY overexpression.
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