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GE29-0005-94

His GraviTrap™ TALON®

Cytiva 29-0005-94, pack of 10 columns

别名:

His GraviTrap

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

包装

pack of 10 columns

制造商/商品名称

Cytiva 29-0005-94

柱床体积

1 mL

色谱柱内径

15 mm

基质

6% cross-linked agarose

粒径

60-160 μm

cleaning

2-14(Co2+-stripped medium)

工作范围

3-12(Co2+-stripped medium)

容量

≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

储存温度

2-8°C

一般描述

His GraviTrap™ Talon® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.

应用

His GraviTrap™ Talon® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap™ Talon® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon® Superflow medium is negligible.

特点和优势

  • Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
  • Ready-to-use gravity flow column prepacked with 1 mL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • One run takes about 30 minutes, and large sample volumes can be applied all at once.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

GraviTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

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商品

Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

实验方案

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

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