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保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
制造商/商品名称
Cytiva 28-4013-53
包装
pkg of 50 x 100 μL
柱床体积
100 μL
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
容量
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
相关类别
一般描述
* See the section for licensing information in our terms and conditions.
Purification of unclarified samples on His SpinTrap™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap™ together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose™ High Performance medium is negligible.
特点和优势
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose™ High Performance in prepacked spin columns.
- Yields up to 750 μg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
储存及稳定性
分析说明
法律信息
商品
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
实验方案
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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