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Merck
CN
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安全信息

P6611

Millipore

HIS-Select® 镍亲和凝胶

(1:1 suspension in a 20% ethanol solution)

别名:

Ni-NTA resin, nickel charged agarose

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.56

偶联物

magnetic beads

质量水平

200

形式

(1:1 suspension in a 20% ethanol solution)

特点

hydrophilic

包装

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

浓度

1.5-2.4 mL/mL (suspension in packed gel)

技术

protein purification: suitable

颜色

faint blue to very dark blue

基质

6% Beaded Agarose

容量

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

转变温度

flash point 32 °C (closed cup)

储存温度

2-8°C

相关类别

一般描述

HIS-Select®镍亲和凝胶强大耐用，可在高流速下捕获带组氨酸标签的重组蛋白。带组氨酸标签的重组蛋白可在天然或变性条件下结合。
HIS-Select®镍亲和凝胶是用于纯化组氨酸标签蛋白的固定化金属离子亲和色谱(IMAC)产品。特制四齿NTA螯合基团与镍琼脂糖珠之间特有的无电荷亲水性连接可确保在蛋白纯化中对小到中等大小的组氨酸标签蛋白呈现高选择性，同时又能降低与其他蛋白的非特异性结合。HIS-Select镍亲和凝胶对组氨酸标签蛋白具有选择性，对其他蛋白则只有较低的非特异性结合。选择性可通过在色谱过程中加入咪唑调节。

应用

HIS-Select®镍亲和凝胶用于纯化重组蛋白，如EF手型钙结合蛋白(S100A14)、 LIM同源框转录因子1α蛋白、sigma-1受体以及harpin蛋白、稳定蛋白1(SP1)和BCR-ABL融合蛋白。

特点和优势

  • 带来更高纯度的高选择性。
  • 特有的非电荷亲水性连接减少非特异性结合。
  • 组氨酸标签蛋白结合容量超过15 mg/mL。
  • 在变性或非变性条件下都能结合。
  • 一步完成纯化。
  • 最大限度减少不必要的离子相互作用。
  • 极低的镍浸出。
  • 粒径：45-165 μm。

联系

它也适用 EZview™ 技术（产品代码 E3528）。

外形

20% 乙醇溶液的混悬液 (1:1)

储存及稳定性

存放恰当的HIS-Select镍亲和凝胶至少可稳定保存一年。HIS-Select镍亲和凝胶在每次用后都应清洗，储存缓冲液中应添加20%乙醇等抗菌剂。

法律信息

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

危险分类

Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

89.6 °F - closed cup

闪点(°C)

32 °C - closed cup

法规信息

危险化学品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Can imidazole be used with HIS-Select® Nickel Affinity Gel, Product P6611?

    For column chromatography, no more than 20 mM is suggested in the extract, equilibration, and wash buffers to prevent non-specific binding of proteins. No more than 250 mM is suggested for the elution buffers.  Many proteins will elute with imidazole levels as low as 100 to 200 mM.  For batch methods the imidazole concentration may have to be reduced or eliminated.When a protein is expressed at low levels, the presence of the imidazole limits the binding of the protein in the batch method but not when  used in a column.

  2. Can Tris buffers be used instead of phosphate buffer for HIS-Select® Nickel Affinity Gel, Product P6611?

    Yes, Tris buffers should work.

  3. Why won't my recombinant protein with a histidine-containing tag bind to HIS-Select® Nickel Affinity Gel, Product P6611?

    Verify the pH and composition of sample and equilibration buffers.  Make sure there are no chelating or reducing agents present in the extraction buffer. If using the batch mode, remove imidazole.  Run the affinity purification under denaturing conditions.  Run a Western blot of the extract to verify that the recombinant protein is present.

  4. Can I use SDS with HIS-Select® Nickel Affinity Gel, Product P6611?

    0.1% SDS has been used with some samples, with no adverse effects on the observed protein binding.  However, SDS will effectively coat proteins and may block the binding to the column.  It is probably very  protein-specific and an SDS concentration that works for one protein may not work for another.

  5. What needs to be done if the HIS-Select® Nickel Affinity Gel, Product P6611, resin turns brown on reuse?

    During purification many protein extracts tend to discolor an affinity gel during the loading step. The original color will return after the wash or elution step. If the color is still not changing strip and recharge the affinity gel with nickel.  Wash with EDTA and recharge with Nickel solution.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Leucine zipper-like motifs of HrpZPss are not essential to induce hypersensitive response in tobacco
Anil K, et al.
Journal of Plant Physiology, 96(1), 57-62 (2014)
Purification and characterization of the guinea pig sigma-1 receptor functionally expressed in Escherichia coli
Ramachandran S, et al.
Protein Expression and Purification, 51(2), 283-292 (2007)
Subramaniam Ramachandran et al.
Protein expression and purification, 51(2), 283-292 (2006-09-12)
Sigma receptors once considered as a class of opioid receptors are now regarded as unique orphan receptors, distinguished by the ability to bind various pharmacological agents such as the progesterone (steroid), haloperidol (anti-psychotic), and drugs of abuse such as cocaine
S100A14, a member of the EF-hand calcium-binding proteins, is overexpressed in breast cancer and acts as a modulator of HER2 signaling
Xu C, et al.
The Journal of Biological Chemistry, 289(2), 827-837 (2014)
F Weerkamp et al.
Leukemia, 23(6), 1106-1117 (2009-04-24)
BCR-ABL fusion proteins show increased signaling through their ABL tyrosine kinase domain, which can be blocked by specific inhibitors, thereby providing effective treatment. This makes detection of BCR-ABL aberrations of utmost importance for diagnosis, classification and treatment of leukemia patients.
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