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Merck
CN

GAHK20

葡萄糖 (HK) 检测试剂盒

sufficient for 20 assays

别名:

葡萄糖定量试剂盒

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关于此项目

UNSPSC代码:
12164500
NACRES:
NA.84

主要文件

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一般描述

Glucose, the most abundant natural monosaccharide, serves asthe universal fuel for living cells. In plants, it accumulates in fruits andother tissues in its free form; in animals, it circulates in the bloodstreamand is taken up by cells to power glycolysis, the pentose-phosphate pathway(providing NADPH for biosynthesis and redox control), and the tricarboxylicacid (TCA) cycle coupled to oxidative phosphorylation to generate ATP.
Quantitative measurement of glucose in serum, plasma, urineor culture media underpins metabolic research (including flux analysis andinsulin-signaling studies), and disease modeling (such as the Warburg effect incancer and investigations into oxidative-stress pathways or glycogen storagedisorders).
The GAHK20 enzymatic glucose assay kit, based on theglucose/hexokinase reaction offers high sensitivity, a broad linear range, andcompatibility with high-throughput screening and detailed mechanistic studiesof metabolic health and disease.

仅试剂盒组分

产品编号
说明
  • glucose (HK) assay reagent 20 mL
  • glucose standard .5 mL

储存分类代码

10 - Combustible liquids

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000462749
0000446643
0000446643
0000411005
0000411005

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  1. How do I determine what sample size to use in the Glucose (HK) Assay Kit, Product GAHK20?

    If you know approximately how much glucose is present, dilute the sample down to 0.5 to 50 ug of glucose and use between 10 to 200 ul sample volume.  If you do not know how much glucose is present, you should assay the undiluted sample and dilutions of it.  Based on the dilution factor you can determine the glucose concentration.

  2. Can the procedure for Glucose (HK) Assay Kit, Product GAHK20, be scaled down?

    Although we have not done it, you should be able to scale down the procedure as long as you have pipettes capable of dispensing accurate ul volume quantities and an instrument that can monitor the 340 nm wavelength.

  3. If my sample for the Glucose (HK) Assay Kit, Product GAHK20, has a lot of protein, how do I deproteinize it?

    You can deproteinize by using barium hydroxide, B4059, and zinc sulfate, Z2876, by adding 0.2 ml of sample to 1.8 ml of water, swirl and mix, then 1.0 ml of barium hydroxide, swirl and mix, then 1.0 ml of zinc sulfate, swirl and mix thoroughly by shaking, and then filter or centrifuge to obtain clear filtrate.

  4. How do I decolorize a solution for use with Product GAHK20, Glucose (HK) Assay Kit?

    You can decolorize a solution by adding a small amount of charcoal to the solution, swirl, then filter or centrifuge to remove charcoal.

  5. How do I degas a solution for use with Product GAHK20, Glucose (HK) Assay Kit?

    You can degas a solution by either putting it under a vacuum or by bubbling nitrogen through it.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Kunst, A., et al.
Methods of Enzymatic Analysis, 163-172 (1984)
Helen H Tai et al.
Plant physiology and biochemistry : PPB, 146, 163-176 (2019-11-23)
Tubers are vegetative reproduction organs formed from underground extensions of the plant stem. Potato tubers are harvested and stored for months. Storage under cold temperatures of 2-4 °C is advantageous for supressing sprouting and diseases. However, development of reducing sugars can
Michael B Jones et al.
G3 (Bethesda, Md.), 10(12), 4679-4689 (2020-10-29)
Atherosclerosis is a polygenic disorder that often affects multiple arteries. Carotid arteries are common sites for evaluating subclinical atherosclerosis, and aortic root is the standard site for quantifying atherosclerosis in mice. We compared genetic control of atherosclerosis between the two
Saheed Imam et al.
BMC systems biology, 5, 116-116 (2011-07-23)
Rhodobacter sphaeroides is one of the best studied purple non-sulfur photosynthetic bacteria and serves as an excellent model for the study of photosynthesis and the metabolic capabilities of this and related facultative organisms. The ability of R. sphaeroides to produce
Bo-Ruei Chen et al.
Experimental gerontology, 44(8), 493-502 (2009-05-05)
We describe a new chronological lifespan (CLS) assay for the yeast Schizosaccharomyces pombe. Yeast CLS assays monitor the loss of cell viability in a culture over time, and this new assay shows a continuous decline in viability without detectable regrowth
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