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Merck
CN
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文件

安全信息

GAHK20

Supelco

葡萄糖 (HK) 检测试剂盒

sufficient for 20 assays

别名:

Glucose Quantification Kit

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About This Item

UNSPSC代码:
12164500
NACRES:
NA.84

用途

sufficient for 20 assays

质量水平

100

分析物化学类别

sugars (glucose)

技术

photometry: suitable

应用

food and beverages
general analytical

储存温度

2-8°C

相关类别

应用

本试剂盒适于酶促定量测定食物及其他材料中的葡萄糖水平。 首先通过己糖激酶催化三磷酸腺苷(ATP)磷酸化葡萄糖。 随后，在存在氧化型烟酰胺腺嘌呤二核苷酸(NAD)的条件下，葡萄糖-6-磷酸(G6P)通过葡萄糖-6-磷酸脱氢酶(G6PDH)催化的反应磷酸化为6-磷酸-葡萄糖酸。 在这一氧化反应中，相等摩尔数量的NAD被还原为NADH。 相应增加的340 nm吸光值即与葡萄糖浓度直接成正比。

仅试剂盒组分

产品编号
说明
  • glucose (HK) assay reagent 20 mL
  • glucose standard .5 mL

WGK

WGK 3

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. How do I determine what sample size to use in the Glucose (HK) Assay Kit, Product GAHK20?

    If you know approximately how much glucose is present, dilute the sample down to 0.5 to 50 ug of glucose and use between 10 to 200 ul sample volume.  If you do not know how much glucose is present, you should assay the undiluted sample and dilutions of it.  Based on the dilution factor you can determine the glucose concentration.

  2. Can the procedure for Glucose (HK) Assay Kit, Product GAHK20, be scaled down?

    Although we have not done it, you should be able to scale down the procedure as long as you have pipettes capable of dispensing accurate ul volume quantities and an instrument that can monitor the 340 nm wavelength.

  3. If my sample for the Glucose (HK) Assay Kit, Product GAHK20, has a lot of protein, how do I deproteinize it?

    You can deproteinize by using barium hydroxide, B4059, and zinc sulfate, Z2876, by adding 0.2 ml of sample to 1.8 ml of water, swirl and mix, then 1.0 ml of barium hydroxide, swirl and mix, then 1.0 ml of zinc sulfate, swirl and mix thoroughly by shaking, and then filter or centrifuge to obtain clear filtrate.

  4. How do I decolorize a solution for use with Product GAHK20, Glucose (HK) Assay Kit?

    You can decolorize a solution by adding a small amount of charcoal to the solution, swirl, then filter or centrifuge to remove charcoal.

  5. How do I degas a solution for use with Product GAHK20, Glucose (HK) Assay Kit?

    You can degas a solution by either putting it under a vacuum or by bubbling nitrogen through it.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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