用途
sufficient for 20 assays
质量水平
分析物化学类别
sugars (fructose)
技术
photometry: suitable
应用
food and beverages
general analytical
储存温度
2-8°C
应用
本品用于定量酶法测定食物和其他物质中的果糖。己糖激酶催化三磷酸腺苷 (ATP) 磷酰化果糖。磷酸葡萄糖异构酶 (PGI) 将果糖-6-磷酸转化为葡萄糖-6-磷酸。然后葡萄糖六磷酸脱氢酶 (G6PDH) 在烟酰胺腺嘌呤二核苷酸 (NAD) 的存在下催化 6-磷酸葡萄糖 (G6P) 氧化为 6-磷酸葡萄糖酸酯。在此氧化过程中，等摩尔的 NAD 被还原为 NADH。在 340nm 处增加的吸收值与葡萄糖浓度直接成正比。
仅试剂盒组分
产品编号
说明
- fructose standard 500 mg
- glucose assay reagent 1 mL/vial
- phosphoglucose isomerase 1 mL/vial
Official Methods of Analysis of the AOAC, 28-28 (1995)
Mengxuan Fang et al.
Analytical and bioanalytical chemistry, 412(10), 2339-2351 (2020-02-02)
Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branching, and cyclization, as well as the number, position, and stereochemistry of double bonds within their mono- and poly-unsaturated species.
Yifan Chen et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(3), 373-378 (2019-11-19)
Cholesteryl ester (CE) is an ester of cholesterol and fatty acid (FA). Plasma CE reflects complicated metabolisms of cholesterol, phospholipids, lipoproteins, and dietary FAs. An informatics approach could be useful for analysis of the CE species. In this study, two
Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)
Chih-Yu Cheng et al.
FEBS letters, 589(20 Pt B), 3107-3112 (2015-09-04)
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity
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